TrxA基因对类鼻疽伯克霍尔德菌生物学活性的影响OA

Objective:To investigate the biological function of TrxA in Burkholderia pseudomallei(B.pseudomallei),with a focus on exploring the role of TrxA in regulating B.pseudomallei's biological activities.Methods:A TrxA gene deletion mutant(ΔTrxA)was constructed using homologous recombination,and a complemented strain(ΔTrxA∷TrxA)was obtained via elec-troporation.Using the wild-type(WT)B.pseudomallei strain as a control,systematically evaluated differences among the three strains in terms of growth,motility,biofilm formation,antibiotic resistance,oxidative stress tolerance,and infectivity.Specific as-says included:growth curve analysis,point plate and area measurement methods were used to evaluate motility,crystal violet staining was used to detect biofilm formation,drug sensitivity plate was used to test antibiotic susceptibility,the Kirby-Bauer method was used to evaluate oxidative stress tolerance,and colony counting and CCK-8 assays were used to assess bacterial adhe-sion and cytotoxicity to the host cells.Results:Compared to the WT and ΔTrxA∷TrxA strains,the ΔTrxA mutant exhibited sig-nificantly reduced growth and motility(P<0.05),as well as significantly decreased infectivity and cytotoxicity toward the host cells(P<0.05).Regarding biofilm formation,the ΔTrxA strain formed a more robust biofilm than the WT strain at 24 hour.However,by the 72 hour,no statistical significant differences were observed among the three strains(P>0.05).The ΔTrxA strain showed increased resistance to certain antibiotics,while there were no statistical significant differences in tolerance to oxidative stress in-duced by hydrogen peroxide among all three strains(P>0.05).These findings suggested that TrxA plays a critical role in B.pseu-domallei's biological activity,as well as infectivity and cytotoxic against the host cells.Conclusion:TrxA plays a key role in regu-lating the growth,motility,biofilm formation,infectivity,and cytotoxicity of B.pseudomallei.This study provides a theoretical basis for further elucidating the pathogenic mechanisms of B.pseudomallei and developing novel therapeutic targets.