首页|期刊导航|贵州农业科学|发酵对叶用芥菜膳食纤维结构特征和理化功能特性的影响

发酵对叶用芥菜膳食纤维结构特征和理化功能特性的影响OA

Effects of Fermentation on Structural Characteristics and Physicochemical Functional Properties of Dietary Fiber in Leaf Mustard

中文摘要英文摘要

[目的]探明发酵对叶用芥菜膳食纤维结构特征、理化功能特性的影响,为完善酸菜品质标准、提升营养价值及拓展叶用芥菜综合利用提供理论依据.[方法]以叶用芥菜为原料,采用乳酸菌(植物乳杆菌、肠膜明串珠菌)和食用盐分别进行发酵 8 d处理,对发酵前后芥菜不可溶性膳食纤维和可溶性膳食纤维的颜色、单糖组成等结构特征与持水持油能力等理化特性及胆固醇吸附能力等功能特性进行比对分析.[结果]发酵能显著提升可溶性膳食纤维亮度 L*,显著降低其红色 a*、黄色 b*值;使可溶性膳食纤维形成疏松多孔的断层结构,不可溶性膳食纤维原有致密光滑的结构被破坏.发酵后不可溶性膳食纤维中葡萄糖含量由 16.36 mol%显著降至 10.45~12.84 mol%,阿拉伯糖和鼠李糖含量均升高;可溶性膳食纤维中肠膜明串珠菌发酵使葡萄糖含量显著升至 64.03 mol%,半乳糖醛酸含量降至 3.45 mol%.盐、植物乳杆菌、肠膜明串珠菌发酵后不可溶性膳食纤维持水能力和膨胀力显著增强,分别为 16.33 g/g、14.40 g/g、17.33 g/g和 50.33 mL/g、46.33 mL/g、31.67 mL/g,可溶性膳食纤维持油能力显著提升,分别为 28.98 g/g、31.00 g/g、25.09 g/g.发酵后膳食纤维对胆固醇、胆酸钠及葡萄糖的吸附能力均高于未发酵,其中,膳食纤维胆固醇吸附能力在 pH 7.0时均高于pH 2.0,盐、植物乳杆菌、肠膜明串珠菌发酵后不可溶性膳食纤维和可溶性膳食纤维分别为 4.61 mg/g、6.04 mg/g、5.03 mg/g和 20.71 mg/g、28.89 mg/g、24.22 mg/g;葡萄糖、胆酸钠吸附能力不可溶性膳食纤维分别以肠膜明串珠菌、植物乳杆菌发酵处理最高,分别为 205.92 mg/g、27.79 mg/g;可溶性膳食纤维均以盐发酵处理最高,分别为 84.75 mg/g、32.21 mg/g.[结论]发酵能使叶用芥菜膳食纤维形成疏松多孔结构,优化其理化功能特性,其中,乳酸菌发酵改性效果较好,在肠道环境(pH 7.0)吸附作用较强,可作为叶用芥菜膳食纤维绿色高效改性技术.

[Objective]The effects of fermentation on structural characteristics and physicochemical functional properties of dietary fiber in leaf mustard were investigated to provide a theoretical basis for improving the quality standard of pickled mustard,enhancing its nutritional value,and expanding comprehensive utilization of leaf mustard.[Method]The structural characteristics(such as color and monosaccharide composition),physicochemical properties(such as water and oil holding capacity)and functional performance(such as cholesterol adsorption capacity)of insoluble and soluble dietary fibers in leaf mustard fermented with edible salt,Lactobacillus plantarum and Leuconostoc mesenteroides for 8 d were comparatively analyzed.[Result]Fermentation could significantly increase the brightness L* of soluble dietary fiber,significantly reduce its red a* and yellow b* values,cause soluble dietary fiber to form a loose and porous layered structure,and destroy an original dense and smooth structure of insoluble dietary fiber.After fermentation,the glucose content in insoluble dietary fiber decreased from 16.36 mol%to 10.45-12.84 mol%significantly,and the content of arabinose and rhamnose increased.After L.mesenteroides fermentation,the glucose content in soluble dietary fiber increased to 64.03 mol%,and galacturonic acid content decreased to 3.45 mol%.The water holding capacity and swelling capacity of insoluble dietary fiber in leaf mustard fermented with edible salt,L.plantarum and L.mesenteroides reached 16.33 g/g,14.40 g/g and 17.33 g/g,and 50.33 mL/g,46.33 mL/g,and 31.67 mL/g,respectively.The oil holding capacity of soluble dietary fiber in leaf mustard fermented with salt,L.plantarum and L.mesenteroides reached 28.98 g/g,31.00 g/g and 25.09 g/g,respectively.The cholesterol,sodium cholate and glucose adsorption capacity of dietary fiber in fermented leaf mustard was higher than that of unfermented leaf mustard.The cholesterol absorption capacity of dietary fiber in fermented and unfermented leaf mustard at pH 7.0 was higher than that at pH 2.0.The cholesterol absorption capacity of insoluble and soluble dietary fiber in leaf mustard fermented with edible salt,L.plantarum and L.mesenteroides was 4.61 mg/g,6.04 mg/g and 5.03 mg/g,and 20.71 mg/g,28.89 mg/g and 24.22 mg/g,respectively.The highest glucose capacity of insoluble dietary fiber in leaf mustard fermented with L.mesenteroides was 205.92 mg/g,and the highest sodium cholate adsorption capacity of insoluble dietary fiber in leaf mustard fermented with L.plantarum reached 27.79 mg/g.The glucose and sodium cholate adsorption capacity of soluble dietary fiber in leaf mustard fermented with salt reached 84.75 mg/g and 32.21 mg/g,respectively.[Conclusion]Fermentation can cause the dietary fiber in leaf mustard to form a loose and porous structure and optimize its physicochemical functional properties.The modification effect of lactobacillus fermentation is better,and it has a strong adsorption effect in the intestinal environment(pH 7.0).Therefore,the lactobacillus fermentation technology should be used in green and efficient modification of dietary fiber in leaf mustard.

奥宁;陈晓蝶;刘嘉;王堃;董楠;陈志峰;邓英

贵州省园艺研究所,贵州 贵阳 550006||贵州省园艺工程技术中心,贵州 贵阳 550006贵州医科大学 公共卫生与健康学院,贵州 贵阳 561113贵州省园艺研究所,贵州 贵阳 550006||贵州省园艺工程技术中心,贵州 贵阳 550006贵州省园艺研究所,贵州 贵阳 550006||贵州省园艺工程技术中心,贵州 贵阳 550006贵州省园艺研究所,贵州 贵阳 550006||贵州省园艺工程技术中心,贵州 贵阳 550006遵义师范学院 生物与农业科技学院,贵州 遵义 563006贵州省园艺研究所,贵州 贵阳 550006||贵州省园艺工程技术中心,贵州 贵阳 550006

农业科技

叶用芥菜发酵膳食纤维结构理化功能特性植物乳杆菌肠膜明串珠菌

leaf mustardfermentationdietary fiberstructurephysicochemical functional characteristicsLactobacillus plantarumLeuconostoc mesenteroides

《贵州农业科学》 2026 (9)

89-99,11

贵州省农业科学院青年科技基金项目(黔农科一般基金[2025]08)贵州省蔬菜现代农业产业技术体系建设项目(GZCYTX2024-edible salt,01)国家大宗蔬菜产业技术体系贵阳综合试验站项目(CARS-23-G37)贵州省教育厅"百校千企科技攻关揭榜挂帅"(黔科教[2024]016号)贵州省科技计划项目(黔科合服企[2022]005)

10.3969/j.issn.1001-3601.2026.09.010

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