基于QuEChERS-UPLC-MS/MS法的茶叶农药残留检测与分析研究OA

[Objective]The QuEChERS-UPLC-MS/MS method for simultaneously detecting multiple pesticide residues in tea was established to provide technical support for rapid screening of multiple types of pesticide residues in tea.[Method]The pre-treatment conditions of green tea samples were optimized by optimizing the liquid chromatography conditions and mass spectrometry conditions,and combining with the screening experiments of extraction solvents and purification agents,and the established QuEChERS-UPLC-MS/MS method of simultaneously detecting 65 pesticide residues was used to analyze 43 batches of green tea samples.[Result]There was a good linear relationship between the chromatographic peak area and the volume concentration when the volume concentration of 65 pesticides was 0.001-1.000 mg/L.The R2 values all were greater than 0.990 0(except fluoroglycofen)and the quantitative limits were all 5 μg/kg.At three concentration levels of 5 μg/kg,25 μg/kg,and 250 μg/kg,the recovery rates of 65 pesticides ranged from 71%to 120%,and the relative standard deviations were 0.98%-19.63%,which were lower than the 20%specified in NY/T 788-2018 and met the requirements.A total of 10 pesticides were detected in 43 batches of tea samples,and the detected content ranged from 0.001 to 0.012 mg/kg.Among them,acetamiprid had the highest detection rate(14.0%),and was detected in 6 batches of tea samples.Buprofezin had the second highest detection rate(9.3%),and was detected in 4 batches of tea samples.The pesticide residues detected in tested tea samples all were below the maximum residue limit standard for pesticides in tea.[Conclusion]The precision,accuracy and sensitivity of the pesticide residue detection method based on QuEChERS-UPLC-MS/MS all meet the requirements for pesticide residue detection analysis,and it can simply and quickly detect various pesticide residues in tea.