栽培种与野生种辣椒T2T基因组的GH3基因家族鉴定OA

[Objective]The structural and functional characteristics of GH3 gene family members in pepper were investigated to provide a theoretical basis and references for research on genes related to growing development and stress resistance of pepper.[Method]The members of GH3 gene family were identified by using bioinformatics based on T2T genomes of Capsicum annuum and Capsicum rhomboideum.Their physicochemical properties,gene structure,promoters,protein three-dimensional structure,phylogenetic relationship,temporal and spatial expression patterns and molecular docking simulation were analyzed.[Result]Fourteen CaGH3 genes were distributed on four chromosomes,and ten CrGH3 genes were distributed on six chromosomes.Evolutionary analysis showed that GH3 gene members were divided into two subgroups.Gene duplication analysis showed that fragment duplication had a significant impact on amplification of GH3 genes in pepper.The GH3 promoters in C.annuum contained abundant light-responsive elements compared with C.rhomboideum.The spatio-temporal expression analysis revealed that CaGH3.7 maintained a relatively high expression level in all tissues and at fruit development stage,while CaGH3.13 reached its peak expression level in leaf.The molecular docking simulation showed that CaGH3.7 and CrGH3.6 had a strong binding potential with jasmonic acid(JA-Ile).[Conclusion]There are differences in chromosome distribution,promoter elements and expression pattern among the identified members of GH3 gene family based on T2T genomes of C.annuum and C.rhomboideum.CaGH3.7 may play an important role at each developmental stage of pepper,and GH3 genes may be involved in jasmonic acid signal transduction.