黄花棘豆中1个新的黄酮苷化合物及生物活性研究OA

To investigate the chemical constituents of Oxytropis ochrocephala and their antioxidant activities,the ethanol extract of O.ochrocephala was separated and purified using chromatographic techniques including silica gel column chromatography,Sephadex LH-20 chromatography and semi-preparative high-performance liquid chromatography.The structures of the isolated compounds were elucidated by comprehensive spectroscopic analyses,including UV,IR,HR-MS and NMR.The antioxidant activities of the isolated compounds were evaluated using 1,1-diphenyl-2-picrylhydrazyl(DPPH)and 2,2-azinobis-(3-ethyl-benzothiazole-6-sulfonic acid)(ABTS)radical scavenging assays.The results were as follows:(1)Twelve compounds were isolated and identified as ochrocephalamine Q(1),(6aR,11aR)-10-hydroxy-3,9-dimethoxypterocarpan(2),3',7-dihydroxy-2',4'-dimethoxyisoflavan(3),bosenegaloside A(4),5-hydroxy-2-(4-hydroxyphenyl)-7-methoxy-3-[6-O-(1-oxohexadecyl)-α-D-altropyranosyl]-4H-1-benzopyran-4-one(5),rhamnocitrin(6),3,5,7-trihydroxy-4'-methoxyflavonol(7),3-O-rhamnocitrin-6-O-benzoyl-β-D-glucopyranoside(8),rhamnocitrin-3-O-β-D-galactoside(9),isoquercitrin(10),rhamnetin 3-O-β-D-galactoside(11)and rhamnocitrin 3-O-β-D-glucoside(12).Among these,Compound 1 was a new compound,while compounds 2,4 and 5 were reported in this species for the first time.(2)Compounds 6,10 and 11 exhibited notable antioxidant activities in the DPPH radical scavenging assay,with IC50 values of(4.90±0.35),(0.80±0.06)and(1.07±0.02)μg·mL-1,respectively,which were stronger than that of the positive control vitamin C[IC50 value of(5.72±0.52)μg·mL-1].In addition,in the ABTS radical scavenging assay,Compound 11 also showed strong antioxidant activity,with an IC50 value of(0.32±0.05)μg·mL-1.These findings further enrich the chemical constituents of O.ochrocephala and provide valuable information for its potential resource development and utilization.