小麦DUF292基因家族鉴定及表达模式分析OA

To mine key stress-resistant genes in common wheat(Triticum aestivum),this study performed genome-wide identification and functional analysis of TaDUF292 gene family using bioinformatics approaches.The results were as follows:(1)A total of 33 TaDUF292 members were identified in common wheat and designated TaDUF292-01 to TaDUF292-33,which distributed unevenly on 15 chromosomes.(2)All proteins encoded by the TaDUF292 family were hydrophilic proteins and were mainly localized in chloroplasts,mitochondria and nuclei.(3)Phylogenetic analysis divided the TaDUF292 family into three evolutionary clades,and genes in the same clade exhibited consistent gene structures and conserved motifs.(4)Cis-acting elements in promoters were dominated by light-responsive and hormone regulatory elements,which indicated that this gene family participated in the integration of plant environmental signals.(5)miRNA target prediction revealed that 16 types of cleavage-inhibiting miRNAs and 5 types of translation-inhibiting miRNAs jointly constituted a regulatory network.(6)Expression pattern analysis showed that TaDUF292 family genes exhibited obvious tissue-specific expression and differential response patterns to drought,salt,heat and pathogen infection stresses,and several members were significantly upregulated under combined stress conditions.This study systematically clarifies the molecular characteristics and potential function of the TaDUF292 family in common wheat,providing genetic resources and a theoretical basis for stress-resistant molecular breeding of common wheat.