桃花芽休眠期PpPP2C基因鉴定与表达分析OA

To characterize the expression of PP2C gene family members during peach flower bud dormancy,seven PpPP2C genes were identified from transcriptome data of dormant peach flower buds.Bioinformatics methods were used to analyze the physicochemical properties,phylogenetic relationships,conserved motifs,and promoter cis-acting elements,and qRT-PCR was employed to measure PpPP2C expression levels in varieties with differing chilling requirements from pre-dormancy to dormancy release.The results were as follows:(1)The amino acid sequences encoded by the seven PpPP2C genes exhibited highly similar physicochemical properties and conserved motif structures.All seven PpPP2C genes belonged to Subfamily A,a key component of the abscisic acid(ABA)signaling pathway,suggesting their involvement in dormancy regulation through ABA signal transduction.(2)The promoter regions of PpPP2C genes contained abundant abscisic acid,light,and methyl jasminate response elements,which are thought to regulate PP2C transcription during dormancy.(3)qRT-PCR results showed higher PpPP2C expression before dormancy,with levels declining after dormancy onset,indicating that dormancy suppressed PpPP2C expression.However,in varieties with CR≥400 h,this decrease began earlier than in those with CR≤200 h.This discrepancy might be attributed to different periods of dormancy resulting from varying chilling requirements,where varieties with CR≤200 h required shorter day lengths or lower temperature and consequently entered dormancy later.This study preliminarily elucidated the role of PP2C genes in ABA-mediated dormancy regulation,providing an important basis for further analysis of its regulatory network.