茶树DIR基因家族鉴定及在炭疽病胁迫下表达模式分析OA

Dirigent(DIR)proteins play crucial roles in lignin and lignan biosynthesis process,significantly influencing plant growth,development and stress responses.This study identified 40 CsDIR genes through genome-wide screening in Camellia sinensis,and analysed for physicochemical properties,phylogenetic tree,conserved motifs,chromosomal localization,cis-acting element structural features,and gene expression patterns of DIR gene family under tea anthracnose stress.The results were as follows:(1)Phylogenetic analysis showed that the CsDIR genes were divided into three subfamilies,and their promoter regions contained response elements for auxin,abscisic acid,wounding and pathogens.(2)Analysis of protein structures and protein conserved domains confirmed that 40 CsDIRs contained a β-barrel composed of eight antiparallel β-strands.In addition,39 CsDIRs possessed a Dirigent domain,while one contained a Dirigent superfamily domain.(3)Results of chromosomal localization and collinearity analysis indicated that 40 CsDIR genes were distributed across 10 chromosomes and 5 distinct Contigs.Within CsDIR gene family,we identified 12 pairs of tandemly duplicated genes and 4 pairs of collinear genes.(4)Expression pattern analysis of 12 CsDIR genes under tea anthracnose stress demonstrated that all 12 CsDIR genes uniformly responded to tea anthracnose stress.This study provides a foundation for future research on the function of DIR gene family in response to biotic stresses in tea plants.