不同发酵时间与足火干燥工艺对红茶理化品质的影响OA
Effects of different fermentation time and second-drying technologies on physicochemical quality of black tea
[目的]探究不同发酵时间与足火干燥工艺对龙井43茶树品种加工红茶的品质影响,为龙井43茶树品种的高效开发利用及红茶加工工艺优化提供参考依据.[方法]以龙井43品种1芽2叶鲜叶为原料,采用高效液相色谱和顶空固相微萃取—气相色谱—质谱联用技术,对不同发酵时间和足火干燥工艺加工的红茶开展理化成分、儿茶素组分和挥发性成分等的差异分析.[结果]发酵2 h红茶的茶多酚含量为18.41%,茶黄素和可溶性糖含量最高,分别为1.90%和3.03%;足火80℃干燥1.5 h后升温至90℃干燥20 min(LG89)红茶的茶黄素含量为1.22%,茶多酚含量最低(10.53%),可溶性糖含量最高(4.00%).没食子酸、儿茶素组分和可可碱含量随发酵时间的延长而降低.LG89足火干燥工艺对没食子酸、表没食子儿茶素没食子酸酯(EGCG)和酯型儿茶素总量的保留显著高于其他3种足火干燥工艺(P<0.05).主成分分析、正交偏最小二乘判别分析和聚类热图分析结果表明,可溶性糖、茶黄素、咖啡碱、生物碱总量和茶多酚是发酵时间引起红茶品质差异的关键成分,茶黄素、表儿茶素(EC)、茶多酚、EGCG和酯型儿茶素总量是足火干燥工艺引起红茶品质差异的关键成分.不同发酵时间和足火干燥工艺样品均鉴定出关键挥发性成分30种,延长发酵时间,醇类、杂环化合物和酯类等挥发性物质含量增加;发酵2 h,芳樟醇含量为26.05 μg/g,顺式-3-己烯醇含量最高,为2.49 μg/g.足火70℃干燥有利于挥发性物质的保留,30种关键挥发性成分总量、醇类和杂环化合物含量均高于其他3种足火干燥工艺,芳樟醇和2-(5-甲基-5-乙烯基四氢呋喃-2-基)丙-2-基碳酸乙酯含量均最高,分别为25.72和22.90 μg/g.[结论]采用龙井43的1芽2叶鲜叶加工红茶,发酵2 h的红茶滋味和香气品质较佳;足火80℃干燥1.5 h后升温至90℃干燥20 min的红茶滋味较佳;足火70℃干燥的红茶香气较佳.
[Objective]This study aimed to investigate the effects of different fermentation time and second-drying technologies on the quality of black tea processed from the Longjing 43 tea variety,providing reference for efficient de-velopment and utilization of Longjing 43 tea variety,as well as black tea processing technology optimization.[Method]One bud and two leaves of Longjing 43 variety were taken as raw materials.High-performance liquid chromatography and headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry were utilized to investigate the differences in physicochemical components,catechin components,and volatile components of black tea under diffe-rent fermentation time and second-drying technologies.[Result]After 2 h of fermentation,the tea polyphenol content reached 18.41%,with theaflavin and soluble sugar peaking at 1.90%and 3.03%respectively.Under second-drying at 80℃for 1.5 h followed by an increase to 90 ℃ for 20 min(LG89),the theaflavin content of black tea was 1.22%,tea polyphenol was at the lowest(10.53%),and soluble sugar content peaked at 4.00%.Prolonged fermentation resulted in a significant decrease in gallic acid content,catechin component content,and theobromine content.In contrast,the LG89 variable-temperature second-drying technology demonstrated a significantly higher retention of gallic acid,epigallocatechin gallate(EGCG),and total ester catechins compared to the other three second-drying technologies(P<0.05).Principal component analysis,orthogonal partial least squares discriminant analysis,and cluster heatmap analysis revealed that,soluble sugar,theaflavin,caffeine,total alkaloid,and tea polyphenol were the key components contributing to quality differences in black tea due to fermentation time;theaflavin,epicatechin(EC),tea polyphenol,EGCG,and total ester catechins were identified as the key components influencing quality differences in black tea resulting from the second-drying technologies.A total of 30 key aroma components were identified in the samples under different fermentation time and second-drying technologies.With an increase fermentation time,the content of volatile components such as alcohols,heterocyclic compounds,and esters increased;fermentation for 2 h led to linalool content of 26.05 μg/g and the highest content of(3Z)-hex-3-en-1-ol of 2.49 μg/g.The second-drying at 70℃was found to better preserve volatile sub-stances,as total content of 30 key volatile components and contents of alcohols and heterocyclic compounds were higher compared to the other three second-drying technologies.The contents of linalool and ethyl 2-(5-methyl-5-vinyltetrahy-drofuran-2-yl)propan-2-yl carbonate reached the highest,which were 25.72 and 22.90 μg/g respectively.[Conclusion]When using fresh leaves with one bud and two leaves of Longjing 43 to process black tea,the taste and aroma quality fer-mented for 2 h are better;second-drying at 80℃for 1.5 h followed by drying at 90℃for 20 min yields better taste for black tea;second-drying at 70℃leads to better aroma of black tea.
杨留勇;周佳;杜云霄;孙悦;阮龙;王常乐;邓燕莉
毕节职业技术学院农业工程系,贵州 毕节 551700毕节职业技术学院农业工程系,贵州 毕节 551700毕节职业技术学院农业工程系,贵州 毕节 551700毕节职业技术学院农业工程系,贵州 毕节 551700贵州大学茶学院,贵州 贵阳 550025贵州大学茶学院,贵州 贵阳 550025贵州大学茶学院,贵州 贵阳 550025
农业科技
龙井43红茶加工发酵时间足火干燥理化品质挥发性成分
Longjing 43black tea processingfermentation timesecond-dryingphysicochemical qualityvola-tile components
《南方农业学报》 2026 (8)
2388-2401,14
贵州省科技计划项目(黔科合支撑[2026]一般236)毕节市揭榜挂帅项目(毕科合重大专项[2022]5号)毕节市科技计划项目(毕科合平台[2025]20号)毕节职业技术学院茶叶生产与加工技术科研团队项目(BZYKYTD-2024-1) Guizhou Science and Technology Plan Project(QKHZC[2026]Yiban 236)Bijie Challenge-Based Project(BKHZDZX[2022]5)Bijie Science and Technology Plan Project(BKHPT[2025]20)Tea Production and Processing Technology Research Team Project of Bijie Vocational and Technical College(BZYKYTD-2024-1)
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