子宫内膜癌组织SOX7、CSMD1表达水平及与病理特征、预后的关系OA
Expression levels of SOX7 and CSMD1 in endometrial cancer tissues and their associations with pathological characteristics and prognosis
目的 探讨子宫内膜癌(EC)组织 SRY 盒相关基因 7(SOX7)、CUB 和 Sushi 多结构域基因 1(CSMD1)表达水平及与病理特征、预后的关系.方法 选取河北北方学院附属第一医院 2019 年 11 月至 2022 年 1 月收治的 125 例 EC 患者,术中收集EC 组织标本(EC 组)及相对应癌旁组织标本(癌旁组),根据患者预后分为预后不良组(n=52)和预后良好组(n=73);qRT-PCR仪检测 SOX7、CSMD1 mRNA 水平;免疫组化法检测 SOX7、CSMD1 表达;采用 Kaplan-Meier 生存曲线分析患者预后;采用多因素 COX 回归分析影响因素,受试者工作特征(ROC)曲线分析预测价值.结果 EC 组 SOX7、CSMD1 mRNA 表达水平均低于癌旁组,差异有统计学意义(t 值分别为 24.538、24.034,P<0.05);EC 组中 SOX7、CSMD1 蛋白阳性表达率均低于癌旁组,差异有统计学意义(χ2 值分别为 13.581、13.511,P<0.05);EC 组 SOX7、CSMD1 蛋白阴性表达与 FIGO 高分期、低分化、淋巴结转移有关(χ2 值介于 6.108～10.523 之间,P<0.05);SOX7、CSMD1 阳性患者 3 年生存率高于阴性表达患者(χ2 值分别为 4.195、12.755,P<0.05);多因素 COX 回归显示 FIGO 高分期、低分化、淋巴结转移是影响患者预后的危险因素(HR 值分别为 2.304、3.048、3.521,P<0.05),SOX7、CSMD1 mRNA 高表达为保护因素(HR 值分别为 0.324、0.304,P<0.05);ROC 曲线显示SOX7、CSMD1 mRNA 联合预测 EC 患者预后的 AUC 为 0.840,优于单独预测(Z 值分别为 2.715、2.718,P<0.05).结论 子宫内膜癌组织 SOX7、CSMD1 表达水平均异常低表达,二者与患者病理特征及预后有关,联合检测可提高对患者预后的预测价值.
Objective To investigate the expression levels of SRY-box related gene 7(SOX7)and CUB and Sushi multiple domains 1(CSMD1)in endometrial cancer(EC)tissues and their associations with pathological features and prognosis.Methods A total of 125 EC patients admitted to the First Affiliated Hospital of Hebei North University from November 2019 to January 2022 were selected.Intraoperative EC tissue specimens(EC group)and corresponding adjacent non-cancerous tissues specimens(adjacent group)were collected.According to patient prognosis,they were divided into the poor prognosis group(n=52)and the good prognosis group(n=73);qRT-PCR was used to detect SOX7 and CSMD1 mRNA levels;immunohistochemistry was used to detect SOX7 and CSMD1 expression;Kaplan-Meier survival analysis was performed to evaluate patient prognosis;multivariate Cox regression analysis was used to analyze influencing factors,and receiver operating characteristic(ROC)curves were used to evaluate predictive value.Results The mRNA expression levels of SOX7 and CSMD1 in the EC group were lower than those in the adjacent non-cancerous group,and the differences were statistically significant(t=24.538 and 24.034,respectively,P<0.05);the positive protein expression rates of SOX7 and CSMD1 in the EC group were both lower than those in the adjacent non-cancerous group,with statistically significant differences(χ2=13.581 and 13.511,respectively,P<0.05);the negative expression of SOX7 and CSMD1 in the EC group was associated with high FIGO stage,poor differentiation,and lymph node metastasis(χ2-values ranged from 6.108 to 10.523,P<0.05);the 3-year survival rate of patients positive for SOX7 and CSMD1 was higher than that of patients with negative expression(χ2=4.195 and 12.755,respectively,P<0.05);multivariate Cox regression showed that high FIGO stage,low differentiation,and lymph node metastasis were risk factors affecting patient prognosis(HR=2.304,3.048,and 3.521,respectively,P<0.05),while high mRNA expression of SOX7 and CSMD1 were a protective factors(HR=0.324 and 0.304,respectively,P<0.05);ROC curve analysis showed that the AUC for predicting EC patient prognosis using combined SOX7 and CSMD1 mRNA expression was 0.840,which was superior to prediction using either alone(Z=2.715 and 2.718,respectively,P<0.05).Conclusion The expression levels of SOX7 and CSMD1 were abnormally downregulated in EC tissues,which were related to the pathological characteristics and prognosis of patients,and the combined detection could improve the predictive value for patient prognosis.
张娟;刘晓娟;李胜君;梁金玉
河北北方学院附属第一医院妇科,河北 张家口 075000河北北方学院附属第一医院妇科,河北 张家口 075000河北北方学院附属第一医院妇科,河北 张家口 075000河北北方学院附属第一医院妇科,河北 张家口 075000
医药卫生
子宫内膜癌SRY盒相关基因7CUB和Sushi多结构域基因1病理特征预后
endometrial cancerSRY-box transcription factor 7CUB and Sushi multiple domain 1pathological characteristicprognosis
《中国妇幼健康研究》 2026 (9)
15-22,8
张家口市科技局重点研发计划项目(2421082D)
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