BSA-AuNCs荧光传感体系的构建及其对水中Cr(Ⅵ)的可视化检测应用OA

Cr(Ⅵ)is a significant target in the control of water pollution because of its high toxicity and severe carcinogenicity,which makes the development of inexpensive,quick,and simple detec-tion techniques extremely pertinent.In this work,red-fluorescent BSA-gold nanoclusters(BSA-AuNCs)were synthesized using bovine serum albumin(BSA)as a template and L-ascorbic acid as a reducing agent.A new analytical technique for identifying Cr(Ⅵ)in water was developed using these BSA-AuNCs as a fluorescent probe.The resulting assay showed high selectivity,a linear range of 9.00×10-7 to 1.00×10-5 mol/L,and a limit of detection(S/N=3)of 4.90 nmol/L.Mechanistic investi-gations revealed that static quenching and inner filter effect work together to quench the fluorescence of BSA-AuNCs by Cr(Ⅵ).Method validation using real water samples(tap water,the water of Yan-he River,and chromium-containing laboratory wastewater)yielded spike recoveries of 95.8％-101％,demonstrating high accuracy and reliability.Furthermore,a portable test strip based on BSA-AuNCs was fabricated,which enabled the rapid and visual detection of Cr(Ⅵ).Collectively,this study provides a practical and effective strategy for on-site screening and monitoring of Cr(Ⅵ)pollu-tion in water environments.