不同光质对辣椒幼苗生长发育、光合作用及碳代谢的影响OA
Effects of Artificial Lighting on Growth,Photosynthesis,and Carbon Metabolism of Chili Pepper Seedlings
[目的]探究不同光质对辣椒幼苗生长发育、光合作用及碳代谢的影响,为辣椒培育壮苗提供理论依据和技术参数.[方法]以'世纪长龙 2号'辣椒为试验材料,设置 3种红光(R)和蓝光(B)的组合光处理:红光∶蓝光=2∶1(T1处理)、红光∶蓝光=3∶2(T2处理)、红光∶蓝光=3∶1(T3处理),并以白光为对照(CK),于辣椒两叶一心时,8:00至 18:00进行补光处理.研究不同光质对辣椒幼苗株高、茎粗、叶长、叶宽、叶面积、叶片数、叶绿素含量(叶绿素 a、叶绿素 b、叶绿素 a+b)、净光合速率(photosynthetic rate,Pn)、胞间二氧化碳浓度(intercellular carbon dioxide concentration,Ci)、气孔导度(stomatal conductance,Gs)和蒸腾速率(transpiration rate,Tr)、糖含量(果糖、葡萄糖、蔗糖、淀粉含量)及碳代谢关键酶活性(蔗糖合成酶(sucrose synthase,SS)、蔗糖磷酸合成酶(sucrose phosphate synthase,SPS)、中性转化酶(veutral invertase,NI)和可溶性酸性转化酶(soluble acid invertase,AI))的影响.[结果]不同光质处理下辣椒幼苗株高均高于对照,20、30、40 d时,T3处理株高显著高于CK,分别较CK提高26.22%、27.29%和8.49%;30 d时,T2和T3处理株高显著高于CK,较CK分别增加了10.50%和 3.28%.20～40 d时,T1、T2和 T3处理叶长均显著高于 CK,T3处理叶宽、叶面积和叶片数显著高于 CK.3个组合光质下,叶绿素含量均高于对照,其中,T3处理叶绿素 a、叶绿素 b和叶绿素 a+b含量较 CK分别增加14.52%、60.00%和 52.17%.T2处理的净光合速率、胞间二氧化碳浓度和气孔导度均显著高于 CK,较 CK分别提高44.65%、43.45%和 60.00%.T2处理葡萄糖、蔗糖和淀粉含量与 CK相比均最高,分别较 CK增加了 50.14%、49.2%和 34.17%;T3处理中性转化酶显著高于 CK,较 CK提高 21.72%,T1处理蔗糖合成酶显著高于 CK处理,较CK增加 32.29%.[结论]红光∶蓝光=3∶2处理(T2)下辣椒幼苗生长健壮,光合速率大,糖含量高,利于碳水化合物的转化和积累,可作为培育壮苗的有效参数.
[Objective]Effects of artificial light exposure on growth,photosynthesis,and carbon metabolism of chili pepper seedlings were studied.[Method]'Shijichanglong 2'chili pepper plants were grown under the artificial lighting using red(R)and blue(B)lamps in combinations of 2∶1(T1),3∶2(T2)or 3∶1(T3),along with white light as control(CK).When the two leaves of pepper are in one center,add light from 8:00 to 18:00,effects of different light qualities on pepper seedlings,plant height,stem diameter,leaf length,leaf width,leaf area,number of leaves;chlorophyll content(chlorophyll a,chlorophyll b,chlorophyll a+b);net photosynthetic rate(Pn),intercellular carbon dioxide concentration(Ci),stomatal conductance(Gs)and transpiration rate(Tr);effects of sugar content(fructose,glucose,sucrose,starch content)and key enzyme activities of carbon metabolism(sucrose synthase(SS),sucrose phosphate synthase(SPS),neutral invertase(NI)and soluble acid invertase(AI))[Result]Comparing to CK,the lighting of various R+B lamp combinations grew taller seedlings in 20,30,and 40 d.The plants were 26.22%taller under T1,27.29%under T2,and 8.49%under T3.On the 30th day,those under T2 were 10.50%and those under T3,3.28%taller.The seedling leaves were significantly longer in 20-40 d under the combination lighting,and significantly wider,larger,and more numerous under T3.The chlorophyll a,chlorophyll b,and total chlorophyll of the seedlings increased by 14.52%,60.00%,and 52.17%,respectively,under T3,while the net photosynthetic rate,intercellular CO2 concentration,and stomatal conductance became significantly 44.65%,43.45%,and 60.00%,respectively,higher under T2.The contents of glucose,sucrose,and starch peaked with 50.14%,49.20%,and 34.17%,respectively,increases under T2.Meanwhile,the neutral invertase activity rose significantly 21.72%under T3,and the sucrose synthase activity 32.29%under T1.[Conclusion]Chili pepper seedlings grew robustly when exposed to the combined R and B lamps in 3:2 ratio(T2)that boosted photosynthetic rate and sugar content to significantly enhance the carbohydrate transformation and accumulation in the plants.
王翠丽;张晓洋;李琦;汪亮芳;杨义荣
甘肃省农业工程技术研究院,甘肃 兰州,730030甘肃省农业工程技术研究院,甘肃 兰州,730030甘肃省农业科学院生物技术研究所,甘肃 兰州,730070甘肃省农业工程技术研究院,甘肃 兰州,730030甘肃省农业工程技术研究院,甘肃 兰州,730030
农业科技
辣椒红光蓝光生长发育光合作用碳代谢
chili pepperred lightblue lightgrowth and developmentphotosynthesiscarbon metabolism
《福建农业学报》 2026 (6)
682-689,8
甘肃省科技特派员(团)专项(25CXNA004)甘肃省自然科学基金项目(23JRRH0016)
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