玉米生平脐蠕孢菌的产黄青霉病毒鉴定及其对寄主生长和致病性的影响OA
Identification of a chrysovirus in Bipolaris zeicola and its impact on host growth and pathogenicity
[目的]探究玉米生平脐蠕孢菌(Bipolaris zeicola)中病毒的多样性,为玉米圆斑病的生态防控提供理论支持.[方法]从玉米生平脐蠕孢菌菌株 CY56 中提取双链 RNA(dsRNA),对该真菌病毒进行测序和鉴定,并通过生物学试验评估该病毒对其寄主 CY56 生长和致病性的影响.[结果]从 CY56 中分离到 4 条 dsRNA 片段,经序列分析鉴定为一种产黄青霉病毒(chrysovirus),命名为玉米生平脐蠕孢菌产黄青霉病毒 1(Bipolaris zeicola chrysovirus 1,BzCV1).BzCV1 隶属产黄青霉病毒科(Chrysoviridae)β-产黄青霉病毒属(Betachrysovirus),与 Curvularia lunata chrysovirus 1(CuCV1)、Alternaria alternate chryso-virus 1(AaCV1)、Setosphaeria turcica chrysovirus 1(StCV1)和 Bipolaris maydis chrysovirus 2(BmCV2)具有高度同源性,其RNA 依赖性 RNA 聚合酶(RdRP)序列同源性分别为 75.6%、79.4%、84.5%和 87.9%.生物学试验结果表明,CY56 的生长特征和致病性与无病毒对照菌株 CY01 无显著差异.[结论]首次在玉米生平脐蠕孢菌中鉴定出一种产黄青霉病毒,该病毒对寄主生长和致病性无显著影响.
[Objective]The diversity of mycovirus in Bipolaris zeicola was investigated to provide theoretical support for the ecological control of northern corn leaf pot.[Method]Double-stranded RNA(dsRNA)was extracted from B.zeicola strain CY56,followed by sequencing and identification of the mycovirus.Biological experiments were further conducted to evaluate the effects of the mycovirus on the growth and pathogenicity of strain CY56.[Result]Four dsRNA segments were isolated from CY56,and identified as a novel chrysovirus through sequence analysis,named Bipolaris zeicola chrysovirus 1(BzCV1).BzCV1 belonged to the genus Betachrysovir-us within Chrysoviridae,and exhibited high phylogenetic homology with Curvularia lunata chrysovirus 1(CuCV1),Alternaria alter-nate chrysovirus 1(AaCV1),Setosphaeria turcica chrysovirus 1(StCV1),and Bipolaris maydis chrysovirus 2(BmCV2),with RNA dependent RNA polymerase(RdRP)sequence identities of 75.6%,79.4%,84.5%and 87.9%,respectively.Biological ex-periments showed no significant differences in growth characteristics or pathogenicity between CY56 and the virus-free control strain CY01.[Conclusion]A chrysovirus was first indentified from B.zeicola,which exterted insignificant effect on the growth and pathoge-nicity of its host.
陈苗苗;刘卫容;郑郧;杜振国;贾东升;张鑫;彭迪;王有宁;章松柏
长江大学农业农村部长江中游作物绿色高效生产重点实验室(部省共建),湖北 荆州 434025长江大学农业农村部长江中游作物绿色高效生产重点实验室(部省共建),湖北 荆州 434025长江大学农业农村部长江中游作物绿色高效生产重点实验室(部省共建),湖北 荆州 434025福建农林大学植物保护学院,福建 福州 350002福建农林大学植物保护学院,福建 福州 350002湖南省农业科学院杂草生物学及安全防控湖南省重点实验室,湖南 长沙 410125湖南省农业科学院杂草生物学及安全防控湖南省重点实验室,湖南 长沙 410125湖北工程学院特色作物资源利用与品质控制湖北省重点实验室,湖北 孝感 432000长江大学农业农村部长江中游作物绿色高效生产重点实验室(部省共建),湖北 荆州 434025
农业科技
真菌病毒产黄青霉病毒双链 RNA玉米生平脐蠕孢菌致病性
mycoviruschrysovirusdouble-stranded RNABipolaris zeicolapathogenicity
《福建农林大学学报（自然科学版）》 2026 (5)
708-716,9
国家自然科学基金项目(31972243)湖北省自然科学基金项目(2024AFB1015)特色作物资源利用与品质控制湖北省重点实验室开放基金项目(2024K006).
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