单细胞转录组测序技术在植物研究中的应用OA

[Background]Single-cell RNA sequencing(scRNA-seq)enables the analysis of gene expression dynamics and cellular heterogeneity in plants at single-cell resolution,addressing the limitations of bulk transcriptomics in cell-type resolution.As a direct readout of cellular states,scRNA-seq has become an essential tool for studying plant development and cell differentiation.[Pro-gress]Recent advances in sample preparation,sequencing strategies and computational analysis have broadened its application to di-verse tissues and species.High-resolution single-cell atlases have unveiled cell differentiation trajectories and regulatory networks,deepening our understandings of organ development,abiotic stress responses and plant-pathogen interactions.However,technical bottlenecks remain,which include inefficient cell isolation,limited capture of certain cell types and lack of spatial imformation.[Prospect]Future integration with single-nucleus sequencing,multi-omics approaches and spatial transcriptomics is expected to pro-vide a more comprehensive panorama of cellular states and their regulatory networks,providing fundamental support for precise crop improvement and stress-resistance mechanism studies.