截干幼化处理对麻栎离体培养的影响OA

To investigate the rejuvenation effect of stump cutting treatment on the in vitro culture of Quercus acutissima Carr.,newly sprouted shoots from 10-year-old Q.acutissima trees after stump cutting were used as explants,and the effects of ex-plants from different stump cutting heights on morphological indicators of test-tube plantlets were studied.The results showed that at the primary induction stage,the induction rates of explants from 10 cm and 30 cm stump cutting treatments reached 60.59%and 58.75%,respectively,which were significantly higher than those of the non-cutting control.At the subculture proliferation stage,the proliferation coefficient,shoot height and internode length of explants from 10 cm,30 cm and 50 cm stump cutting treatments were all significantly greater than those of the control.In the first subculture cycle,the proliferation coefficients of the three treatments were 96.97%,93.18%and 56.82%higher than the control,the shoot heights were 122.55%,114.71%and 56.86%higher,the internode lengths were 99.60%,101.21%and 63.16%high-er.The 10 cm and 30 cm stump cutting treatments significantly reduced the apical meristem mortality of subcultured plant-lets.After six successive subcultures,the proliferation coefficient,shoot height and internode length of subcultured plant-lets were markedly improved compared with the first subculture,realizing efficient proliferation and stable continuous sub-culture.At the rooting culture stage,plantlets derived from 10 cm and 30 cm stump cutting treatments exhibited superior rooting performance,characterized by abundant thick roots and minimal basal callus.Meanwhile,their rooting rates were significantly higher than the non-cutting control,increasing by 19.16 and 15.00 percentage points respectively.Stump cutting exerted a prominent rejuvenation effect on Q.acutissima,and the rejuvenation efficacy was stronger at positions closer to the tree base.