核-壳型磁性纳米颗粒的制备及其在生物育种中的应用OA

Silicon-hydroxyl magnetic nanoparticles were prepared via a ligand-exchange method on the basis of a solvothermal route,followed by surface modification and functional alteration.The successfully synthesized nanoparticles possess high specific surface area,high surface energy and uniform particle size,which endows them with high water-solubility and fa-vorable biocompatibility simultaneously.In biological-breeding-related experiments,silicon hydroxyl magnetic nanoparti-cles show outstanding performance.They can efficiently isolate pBI121-BpCDF3-GFP plasmid DNA and are applicable to extract high-quality genomic DNA and total RNA from transgenic BpCDF3 materials.The established extraction method presents prominent advantages in plant genomic DNA,RNA and target gene plasmids.It shortens operation time and im-proves nucleic-acid concentration and purity,which provides a reliable technical support for subsequent molecular-biology experiments and supplies innovative materials and solutions for streamlining the experimental procedures of biotechnology-based breeding.