云南栘[木衣]bHLH22基因克隆及表达OA

The bHLH transcription factors play critical regulatory roles in plant growth,development and stress tolerance.To further explore the expression patterns and functional mechanisms of bHLH transcription factors in Docynia delavayi(Franch.)Schneid.,we selected DdebHLH22,a key gene involved in fruit aroma biosynthesis identified via combined multi-omics analysis of genome,transcriptome and metabolome,as the research material.Gene cloning was performed based on its CDS sequence,followed by bioinformatics analysis.The tissue-specific expression of this gene was also detec-ted using RT-qPCR.The results showed that the full-length CDS of DdebHLH22 was 744 bp.This gene encoded a protein of 243 amino acids with a relative molecular mass of 27 800.67 and a theoretical isoelectric point of 5.58.The protein was predicted to be an unstable hydrophilic protein,containing 34 potential protein phosphorylation sites,with no signal pep-tide or transmembrane domain.Its secondary and tertiary structures were mainly composed of α-helices and random coils.Phylogenetic tree analysis revealed that D.delavayi had the closest genetic relationship with Malus domestica.Transcrip-tome data indicated that the expression level of DdebHLH22 varied across different fruit developmental stages and posthar-vest storage periods,its expression peaked at postharvest stage S1 and then decreased gradually.RT-qPCR results demon-strated that DdebHLH22 was expressed in fruits,leaves,roots and stems,and exhibited fruit-specific expression character-istics.