紫花苜蓿MsphyA基因鉴定分析及非生物胁迫下表达OA

Phytochrome A(phyA)is a key receptor that enables plants to perceive far-red light signals and regu-lates their growth,development,and stress responses.To investigate the function of the MsphyA gene and its molecular mechanism in response to abiotic stress in alfalfa(Medicago sativa L.),this study conducted a bioin-formatic analysis of the cloned MsphyA gene and detected its expression patterns under various abiotic stresses using qRT-PCR.Bioinformatic analysis revealed that the MsphyA gene has a CDS length of 3375 bp,encod-ing 1124 amino acids,and possesses typical PAS,GAF,and PHY motifs characteristic of the PHY family.Protein analysis indicated that MsphyA has a relative molecular mass of 124.3 kDa and a theoretical isoelectric point of 6.1.It is classified as an unstable,hydrophilic,non-secretory protein without a signal peptide and lacks transmembrane domains.Subcellular localization prediction suggested that MsphyA is localized to the nucleus.Phylogenetic analysis showed that MsphyA is most closely related to MtphyA from M.truncatula.qRT-PCR results demonstrated that salt,drought,acid,high-temperature,and low-temperature stresses all induced MsphyA expression.These findings indicate that MsphyA is widely involved in the abiotic stress responses in alfalfa.This study provides a foundation for further exploration of the MsphyA gene's function.