花苜蓿CIPK基因家族鉴定及干旱胁迫下的表达分析OA

CIPK(calcineurin B-like interacting protein kinase)is a protein that binds to calcium-ion signal response elements and plays a significant role in plant responses to abiotic stress.In this study,25 CIPK gene family members were identified in the Medicago ruthenica genome through local homology alignment and hidden Markov model searches and were divided into four subfamilies(Group Ⅰ-Group Ⅳ).The remaining members were distributed across seven chromosomes,except for MrCIPK24 and MrCIPK25,which were not assembled on chromosomes.Gene structure and conserved domain analyses indicated that the family members could be divided into two types,intron-rich and intron-lacking,with the majority of the motifs being highly conserved.Expression pattern analysis under drought stress and real-time fluorescent quantitative PCR results showed that expression of the majority of the MrCIPK genes was affected by drought stress,among which the MrCIPK02 gene may have a positive regulatory effect on M.ruthenica's response to drought stress.This study provides theoretical support for subsequent functional studies of the CIPK gene family members in M.ruthenica.