摩氏假单胞菌对盐胁迫番茄种子萌发的影响OA

[Objective]A Pseudomonas mosselli strain PtA1 was previously isolated in our lab.Its salt tolerance and its promoting effect on to-mato seed germination under salt stress were studied,and its application potential in the improvement of saline-alkali soil was explored.[Method]Four concentrations of NaCl(25,50,75,and 100 mmol/L)were used to investigate the growth of PtA1 and its promoting effect on tomato seed germination.[Result]The results showed that PtA1 has extremely high salt tolerance and its growth was not affected under the four salt stress concentrations.Soaking"Hongyangli"tomato seeds in PtA1 bacterial suspension had no effect on germination.Plate experi-ments showed that the germination of the seeds was all inhibited under stress conditions of 25,50,75,and 100 mmol/L NaCl.Seed germina-tion rate decreased at concentrations of 50,75 mmol/L NaCl,and root growth was restricted.Seeds could not germinate under high stress con-dition of 100 mmol/L.After soaking the seeds in PtA1 bacterial suspension with OD600=0.3 for 5 minutes,it effectively relieved the inhibito-ry effect on root growth of seeds under 25 mmol/L NaCl stress conditions.In pot irrigation experiments,the application of P.mosselli PtA1 al-so alleviated the inhibitory effect on germination and growth of tomato seeds under salt stress.[Conclusion]PtA1 has the potential to promote tomato germination and growth under salt stress.