基于巨噬细胞极化探讨四子定喘汤对过敏性哮喘的作用效应OA

Objective To observe the effect of Sizi Dingchuan Decoction on macrophage polarization in ovalbumin(OVA)-induced allergic asthmatic rats.Methods Thirty-six male SD rats were randomly divided into 6 groups:blank control group,asthma group,dexamethasone group,and low-,medium-,and high-dose Sizi Dingchuan Decoction(SZDCT)groups,with 6 rats in each group.The asthmatic rat model was established in all groups except the blank control group via OVA sensitization and challenge.After the successful modeling,low-,medium-,and high-dose SZDCT groups were administered with SZDCT at doses of 3.2,6.4,and 12.8 g/kg,respectively;Dexamethasone group received dexamethasone at dose of 1 mg/kg;blank control and asthma groups were treated with an equivalent volume of normal saline.All treatments were administered once daily for 14 consecutive days.The severity of asthma in rats was assessed using asthma symptom scores and pulmonary function tests[respiratory rate(f)and enhanced pause(Penh)]were conducted;Toluidine blue staining was employed to observe pathological changes in lung tissues;Enzyme-linked immunosorbent assay(ELISA)was employed to determine the serum level of immunoglobulin E(IgE);Immunofluorescence assay was employed to detect the relative fluorescence intensity of macrophage marker(CD86 and CD163).Results Compared with the blank control group,following changes in the asthma group were observed:The asthma symptom scores were significantly increased(P<0.01);Pulmonary function indicators,f and Penh,increased(P<0.01);Toluidine blue staining revealed obvious mast cell infiltration in lung tissues;The serum IgE level was significantly elevated(P<0.01);The relative fluorescence intensity of macrophage marker proteins(CD86 and CD163)in lung tissues,as well as the CD163/CD86 ratio,were significantly increased(P<0.01).Compared with the asthma group,following improvements rats exhibited in SZDCT groups and dexamethasone group:The asthma symptom scores and pulmonary function indicators were improved to varying degrees(P<0.05,P<0.01);Inflammatory cell infiltration in lung tissues was reduced;The serum IgE level was significantly decreased(P<0.01);The relative fluorescence intensity of macrophage markers(CD86 and CD163)and the CD163/CD86 ratio in lung tissue were significantly reduced(P<0.05,P<0.01).Conclusion SZDCT exerts a therapeutic effect on OVA-induced allergic asthma in rats,and its mechanism of action may be achieved by inhibiting pulmonary macrophage polarization.