黄精人参方对拟人不良生活方式致亚健康模型大鼠免疫功能的影响OA

Objective:To investigate the effects of Huangjing Renshen Formula(黄精人参方)on immune function of sub-healthy rats induced by anthropomorphic unhealthy lifestyle.Methods:40 rats were randomly divided into a nor-mal group,a model group,and Huangjing Renshen Formula groups of 0.3,0.6,and 1.2 g/kg doses.A rat model of sub-health was established by stimulation using various unpredictable chronic stress factors for 8 weeks,and the rats in the Huangjing Renshen Formula groups of different doses were administered at corresponding doses for 3 weeks.The behav-ioral parameters,complete blood count,the level of complement,the ratio of T cell subset(Tc/Th),and sIgA content in serum and intestinal mucosa were detected.The pathological changes of spleen,thymus,and intestine tissue were ob-served,and the positive expression of ZO-1 and occludin proteins in intestinal tissue was detected by immunohistochemis-try.Results:Compared with the normal group,the model group showed significantly reduced body weight,grip strength,voluntary activity,peripheral blood levels of WBC,LYMPH%,RBC,and HGB,complement C3 and C4 content,Th/Tc ra-tio,content of serum and intestinal mucosal sIgA,weights and organ indices of spleen and thymus,villus length of intesti-nal tissue,V/C ratio,and expression levels of ZO-1 and occludin proteins(P＜0.05 or P＜0.01).In addition,the heart rate,Tc cell proportion,and intestinal tissue crypt depth were significantly increased(P＜0.05).Compared with the model group,the Huangjing Renshen Formula group of the 0.6 g/kg dose exhibited significantly increased weight and RBC level of rats and a reduced Tc cell proportion.The group of the 1.2 g/kg dose had significantly reduced heart rate and duodenal crypt depth and increased voluntary activity,WBC,intestinal sIgA,and ileal villus length(P＜0.05 or P＜0.01).The groups of the 0.3 g/kg and 1.2 g/kg doses presented a significantly increased proportion of Th cells(P＜0.05).The groups of the 0.6 g/kg and 1.2 g/kg doses showed increased LYMPH%,HGB,Th/Tc ratio,serum sIgA lev-els,decreased depth of the ileal crypt,and an enhanced V/C ratio(P＜0.05 or P＜0.01).In addition,Huangjing Rensh-en Formula at all doses could increase the grip strength,complement content,immune organ weights and organ indices,duodenal villus length,V/C ratio,and positive expression of ZO-1 and occludin proteins in rat intestinal tissue(P＜0.05 or P＜0.01).Conclusion:Huangjing Renshen Formula can enhance the immune function of the sub-health model rats induced by anthropomorphic unhealthy lifestyle,which is mainly manifested in increasing the levels of lymphocytes,regu-latory T cells,and complement,and improving intestinal mucosal immune function.