基于UPLC-QTOF-MS对17种深色水果中特征花色苷的研究OA
Characterization of Anthocyanins in 17 Different Dark Fruits by UPLC-QTOF-MS
目的:建立基于超高效液相色谱-四极杆飞行时间质谱(Ultra Performance Liquid Chromatograph-Quadrupole Time-of-Flight Mass Spectrometer,UPLC-QTOF-MS)的标准化分析方法,系统解析17种常见深色水果中花色苷的组成特征,为深色水果花色苷的成分研究、质量控制及数据库构建提供可靠数据支持.方法:以蓝莓、黑枸杞、蔓越莓等17种天然水果为研究对象,采用UPLC-QTOF-MS结合紫外光谱检测技术,在统一实验条件下对花色苷进行定性分析.通过UPLC实现高效分离,QTOF-MS获取精确分子量及特征碎片离子信息(如苷元子离子峰),结合保留时间与文献比对,完成花色苷的结构鉴定.结果:共鉴定出33种花色苷,所有检出成分均以矢车菊素、飞燕草素、矮牵牛素、芍药素、天竺葵素和锦葵素为苷元.其中,矢车菊素-3-O-葡糖苷在11种深色水果中被检出,表明其为深色水果中最普遍的花色苷.蓝莓与越橘含有5种苷元.为花色苷组成最丰富的品种.此外,总结了反相UPLC条件下花色苷的洗脱规律:相同糖基修饰下,苷元保留时间顺序为飞燕草素<矢车菊素<矮牵牛素<天竺葵素<芍药素<锦葵素;相同苷元结构下,糖苷保留时间顺序为半乳糖苷<葡萄糖苷<阿拉伯糖苷,与文献报道一致.定量检测17种深色水果,总花色苷含量范围在0.19%~5.88%之间,其中越橘总花色苷含量最高(5.88%±0.55%).结论:本研究在标准化条件下获得了17种深色水果的花色苷UPLC指纹图谱,明确了其主要组分及结构特征,初步建立了深色水果花色苷的化学轮廓,为花色苷类产品的质量评价、品种鉴别与溯源提供了技术依据.
[Objective]To establish a standardized analytical method based on ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry(UPLC-QTOF-MS)for systematic profiling of anthocyanin composition in 17 common dark fruits,providing reliable data for anthocyanin research,quality control,and database development.[Method]Seventeen natural fruits species,including blueberry,black goji,and cranberry,were analyzed under unified experimental conditions.Anthocyanins were separated by UPLC and characterized using QTOF-MS for accurate molecular weights and diagnostic fragment ions(e.g.,aglycone product ions),combined with UV-Vis spectral data.Structural identification was achieved by comparing retention times and mass spectra with reference data from the literature.[Result]A total of 35 anthocyanins were identified,all derived from six common aglycones:cyanidin,delphinidin,petunidin,peonidin,pelargonidin,and malvidin.Cyanidin-3-O-glucoside was detected in 11 berry species,confirming its status as the most prevalent anthocyanin.Blueberry and bilberry contained all six aglycone types,exhibiting the most diverse anthocyanin profiles.Furthermore,elution trends under reversed-phase UPLC were established:for anthocyanins with identical glycosylation,the retention order of aglycones was delphinidin<cyanidin<petunidin<pelargonidin<peonidin<malvidin;for those with the same aglycone,the retention order of glycosides was galactoside<glucoside<arabinoside,consistent with previously reported data.The total anthocyanin content of 17 dark fruits ranged from 0.19%to 5.88%,and the total anthocyanin content of blueberry was the highest(5.88%±0.55%).[Conclusion]This study presents UPLC fingerprint profiles of anthocyanins in 17 fruit species under standardized conditions,clarifying their major components and structural characteristics.The results provide a foundational chemical profile of berry anthocyanins,supporting quality assessment,varietal authentication,and traceability of berry-derived products.
周慧吉;张佳伟;李廷钊;李波
安利(上海)科技发展有限公司,上海 201203安利(上海)科技发展有限公司,上海 201203安利(上海)科技发展有限公司,上海 201203||安利(中国)植物研发中心,江苏 无锡 214145安利(上海)科技发展有限公司,上海 201203||安利(中国)植物研发中心,江苏 无锡 214145
花色苷超高效液相色谱-四极杆飞行时间质谱(UPLC-QTOF-MS)新鲜深色水果
anthocyaninsUltra Performance Liquid Chromatograph-Quadrupole Time-of-Flight Mass Spectrometer(UPLC-QTOF-MS)berry species
《中国食物与营养》 2026 (8)
26-43,18
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