纤维素酶添加剂对饿蚂蝗干草营养品质及真菌群落的影响OA

To investigate the effects of different cellulase additive levels on the nutritional quality of Desmo-dium multiflorum hay and the fungal community after 30 d of storage,the aboveground tender stems and leaves of D.multiflorum were used as materials,and five cellulase addition levels(0,25,50,75,and 100 mg/kg)were set up.Nutrient composition was determined at 0,30,and 60 d of storage,and fungal community analy-sis was performed on samples stored for 30 d.The results showed that cellulase addition had extremely signifi-cant effects on the contents of soluble sugar,crude protein,ether extract,and crude ash(P<0.01),a signifi-cant effect on neutral detergent fiber content(P<0.05),and no significant effect on acid detergent fiber and tan-nin contents.Overall,cellulase addition increased crude protein content and decreased neutral detergent fiber content,whereas soluble sugar,ether extract,and crude ash contents fluctuated to varying degrees among cellu-lase addition levels.Storage time had extremely significant effects on ether extract,crude ash,and tannin con-tents(P<0.01),and significant effects on neutral detergent fiber and acid detergent fiber contents(P<0.05).With prolonged storage,ether extract content decreased,while crude ash,neutral detergent fiber,acid deter-gent fiber,and tannin contents increased.After 30 d of storage,Ascomycota and Basidiomycota were the domi-nant fungal phyla in all treatments,and the predominant genera included Cladosporium,Cercospora,Remoti-didymella,Vishniacozyma,Setophoma,and Fusarium,with certain differences in relative abundance among cellulase addition levels.The fungal species richness in the 75 mg/kg treatment was significantly lower than that in the control(P<0.05),while the other α-diversity indices showed no significant differences from the con-trol.Grey relational analysis based on nutrient composition and fungal community characteristics showed that the 25 mg/kg cellulase treatment had the highest equal-weight relational degree,indicating a relatively superior com-prehensive evaluation.