发光细菌与刃天青荧光探针反应协同的含汞污染检测OA

An innovative strategy based on the synergistic detection of luminescent bacteria and resazurin fluorescent probe reaction was proposed,and an integrated analysis system of"initial screening precision measurement"was constructed.This method first utilizes the luminescence inhibition effect of mercury ions on luminescent bacteria such as Vibrio fischeri to achieve rapid initial screening.Then,resazurin is introduced as a metabolic activity indicator probe,and its fluorescence intensity change at 590 nm is measured to achieve high selectivity and sensitivity quantitative confirmation of mercury ions.The experimental results showed that the constructed combination method effectively expanded the detection linear range(1~50 µg/L,R2=0.999),with a detection limit as low as 0.05 µg/L and a spiked recovery rate of 96%~100%,relative standard deviation(RSD)＜4%,The effect concentration 50(EC50)is 4.2 µg/L,which combines the advantages of wide coverage,high accuracy,and good repeatability.In addition,this method has good anti-interference ability and exhibits high selectivity for common coexisting heavy metal ions such as Cu2+,Cd2+,Pb2+,Zn2+,etc.This study provides a new approach for the analysis of mercury pollution in water bodies that is biochemical synergistic,fast,reliable,and suitable for complex environmental samples.