VC、VE、GABA对北方寒地克氏原螯虾抗低温应激的影响OA
Effects of VC,VE,and GABA on cold stress resistance in Procambarus clarkii from northern cold regions
试验旨在探究维生素C(VC)、维生素E(VE)和γ-氨基丁酸(GABA)对克氏原螯虾幼虾抗低温应激能力的影响.挑选120尾体质健康、附肢完整的克氏原螯虾,随机分为4组,每组3个重复,每个重复10尾.对照组饲喂基础饲料,试验组分别在基础饲料中添加0.05%VC(VC组)、0.05%VE(VE组)和0.05%GABA(GABA组).试验第1周结束后模拟低温应激,预试期1 w,正式试验期6 w.结果显示,VC组第3周体重显著高于对照组(P<0.05),所有试验组第4周体重显著高于对照组(P<0.05),GABA组第5周和第6周体重显著高于其他组(P<0.05).所有试验组增重率和特定生长率显著高于对照组(P<0.05),VC组和GABA组存活率显著高于对照组(P<0.05).GABA组肝胰腺脂肪酶和胰蛋白酶活性显著高于对照组(P<0.05).低温应激后24 h及试验结束时,所有试验组肝胰腺免疫与抗氧化指标均优于对照组,其中VC组和GABA组的改善效果优于VE组.研究表明,在饲料中添加VC、VE和GABA均可有效缓解低温应激、促进克氏原螯虾幼虾生长恢复,其中GABA和VC在改善生长性能、消化酶活性及免疫抗氧化能力方面整体优于VE.
The experiment aimed to investigate the effects of vitamin C(VC),vitamin E(VE),and γ-aminobutyric acid(GABA)on the cold stress resistance ability of juvenile Procambarus clarkii.A total of 120 healthy juvenile Procambarus clarkii with intact appendages were randomly divided into four groups,with three replicates per group and 10 Procambarus clarkii per replicate.The control group was fed a basal diet,while the experimental groups were fed the basal diet supplemented with 0.05%VC(group VC),0.05%VE(group VE),and 0.05%GABA(group GABA),respectively.After the first week of the experiment,simulated cold stress was applied,the pre-test period was one week,and the formal test period was six weeks.The results showed that the body weight in the group VC was significantly higher than that in the control group in the 3rd week(P<0.05),the body weight in all experimental groups was significantly higher than that in the control group in the 4th week(P<0.05),and the body weight in the group GABA was significantly higher than that in the other groups in the 5th and 6th weeks(P<0.05).The weight gain rate and specific growth rate in all experimental groups were significantly higher than those in the control group(P<0.05),and the survival rates in the groups VC and GABA were significantly higher than those in the control group(P<0.05).The hepatopancreatic lipase and trypsin activities in the group GABA were significantly higher than those in the control group(P<0.05).At 24 hours after cold stress and at the end of the experiment,the hepatopancreatic immune and antioxidant indices in all experimental groups were superior to those in the control group,with the groups VC and GABA showing greater improvements than the group VE.The study shows that dietary supplementation with VC,VE,and GABA can effectively alleviate cold stress and promote growth recovery in juvenile Procambarus clarkii,with GABA and VC being superior to VE in improving growth performance,digestive enzyme activities,and immune and antioxidant capacity.
原韬;牛彦波;吴皓琼;王笑庸;樊川;安琦
黑龙江省科学院微生物研究所,黑龙江 哈尔滨 150010黑龙江省科学院微生物研究所,黑龙江 哈尔滨 150010黑龙江省科学院微生物研究所,黑龙江 哈尔滨 150010黑龙江省科学院微生物研究所,黑龙江 哈尔滨 150010黑龙江省科学院微生物研究所,黑龙江 哈尔滨 150010黑龙江省科学院微生物研究所,黑龙江 哈尔滨 150010
农业科技
克氏原螯虾VCVEGABA低温应激寒地养殖生长性能
Procambarus clarkiiVCVEGABAcold stresscold-region aquaculturegrowth performance
《饲料研究》 2026 (14)
60-64,5
黑龙江省科学院重大攻关项目(项目编号:ZDGG2024SW01)
评论