慢性PM2.5暴露致心脏代谢功能失调的昼夜节律机制OA

[Background]Long-term exposure to ambient fine particulate matter(PM2.5)is a significant risk factor for cardiometabolic disorders.However,the mechanisms of its interaction with the endogenous circadian system remain incompletely understood. [Objective]To investigate whether chronic PM2.5 exposure interferes with the rhythmic expression of the cardiac circadian clock,thereby disrupting downstream antioxidant defenses and metabolic homeostasis,and ultimately driving cardiometabolic dysfunction. [Methods]Seventy-two male C57BL/6 mice were randomly divided into a PM2.5 exposure group(PM group)and a filtered air control group(FA group).Whole-body exposure was conducted for 8 weeks in a meteorological environmental animal exposure system.Samples were collected at six distinct zeitgeber time(ZT)points post-exposure.The 24 h ambulatory blood pressure and serum lipid profiles were monitored.Rhythm parameters were derived via cosinor analysis to compare differences in Midline statistic of rhythm(Mesor),ampli-tude,and phase between the two groups.The rhythmic expression of core circadian clock genes and antioxidant genes in the myocardium was detected by quantitative polymerase chain reaction(qPCR).Myocardial reactive oxygen species(ROS)levels and downstream pathway protein expression were analyzed by immunofluorescence and Western blot(WB),respectively.The expression changes of the clock gene retinoic acid receptor-related orphan receptor α(RORα)were assessed at both the mRNA and protein levels.Finally,Spearman cor-relation analysis was used to explore the relationships among myocardial RORα expression,lipid profiles,and oxidative stress indicators. [Results]Compared to the FA group,mice in the PM group exhibited a blunted circadian rhythm in blood pressure,characterized by sus-tained elevation throughout the day.Chronic PM2.5 exposure showed a significant interaction with ZT on systolic blood pressure(SBP),di-astolic blood pressure(DBP),and mean arterial pressure(MAP)(F-interaction=9.11,5.70,and 6.02,respectively;P<0.05),as well as on serum triglycerides(TG),total cholesterol(T-CHO),low-density lipoprotein cholesterol(LDL-C),and high-density lipoprotein cholesterol(HDL-C)(F-interaction=16.32,11.12,15.39,and 28.09,respectively;P<0.05).Cosinor analysis further revealed that the Mesor values of T-CHO,TG,and LDL-C were significantly increased(P<0.05),while that of HDL-C was significantly decreased in the PM group(P<0.05).The oscillation amplitudes of SBP,DBP,and MAP showed a decreasing trend,whereas those of TG and LDL-C were significantly increased(P<0.05).Furthermore,SBP,T-CHO,and HDL-C all exhibited a significant phase delay(P<0.05).Mechanistically,PM2.5 exposure significantly suppressed the expression of the positive circadian regulator RORα in the myocardium,leading to disordered rhythmic expression of core clock genes(Bmal1,Clock,Per1/2,and Cry1/2).This exposure also inhibited the rhythmic expression of antioxidant genes(GPX1,SOD2,and CAT),resulting in increased ROS generation and elevated expression of calcium/calmodulin-dependent protein kinase II(CaMKII)and reduced nicotinamide adenine dinucleotide phosphate(NADPH)proteins.Correlation analysis further revealed that my-ocardial RORα expression level was negatively correlated with T-CHO,TG,and LDL-C(r=-0.55,-0.63,and-0.51,respectively;P<0.001),and positively correlated with HDL-C(r=0.37,P=0.010),and antioxidant genes GPX1,SOD2,and CAT expression(r=0.34,0.35,and 0.56,respectively;P<0.001). [Conclusion]Chronic PM2.5 exposure induces cardiometabolic dysfunction by suppressing myocardial RORα expression.This suppression disrupts the cardiac circadian clock and the diurnal balance of oxidative stress,triggering oxidative damage and elevating expression of CaMKII/NADPH pathway proteins.Collectively,these alterations precipitate the loss of cardiac metabolic rhythms and subsequent func-tional impairment.