ZrMOF-Fc纳米粒子介导的微波热-动力治疗对口腔鳞状细胞癌Cal-27细胞的杀伤作用OA

Background Microwave thermal-dynamic therapy is a promising alternative treatment for oral cancer,but currently,research on it is extremely limited.Objective To investigate the role of ferrocene-doped zirconium-based metal-organic framework nanoparticles(ZrMOF-Fc NPs)in enhancing microwave thermal-dynamic therapy and elucidate their cytotoxic effects on Cal-27 oral squamous cell carcinoma cells.Methods Dynamic light scattering(DLS),scanning electron microscopy(SEM),transmission electron microscopy(TEM),X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR),and energy-dispersive X-ray spectroscopy(EDS)elemental mapping were used for physicochemical characterization.A near-infrared thermal imaging system was used to monitor temperature changes of ZrMOF-Fc NPs at different concentrations(1,3,5 mg/mL)under microwave irradiation.The 3,3′,5,5′-tetramethylbenzidine(TMB)colorimetric assay was used to evaluate hydroxyl radical generation with or without microwave irradiation and under microwave irradiation at different concentrations of ZrMOF-Fc NPs(0,5,10,15,20 μg/mL).Cell Counting Kit-8(CCK-8)and live/dead cell staining were used to assess the viability of Cal-27 and HOK cells treated with different concentrations of ZrMOF-Fc NPs(0,25,50,100,200 μg/mL).Cal-27 cells were divided into a control group,a microwave group,and a microwave+ZrMOF-Fc group(25,50,100,200 μg/mL);CCK-8 was used to determine cell viability in each group.Live/dead cell staining was performed at 200 μg/mL for validation,and DCFH-DA fluorescence was used to measure intracellular reactive oxygen species(ROS)levels.Results ZrMOF-Fc NPs were quasi-spherical with an average diameter of(269.3±0.5)nm.Under microwave irradiation,the solution temperature increased markedly and rose with increasing concentration.Compared with the non-microwave group,microwave irradiation significantly enhanced the TMB reaction,and the enhancement increased with higher material concentrations.In Cal-27 and HOK cells,there was no significant difference in viability between the 25-200 μg/mL groups and the 0 μg/mL group(P＞0.05).In the treatment experiment,the 200 μg/mL+microwave group showed the most pronounced decrease in viability(P＜0.001);at 200 μg/mL,the microwave+ZrMOF-Fc group exhibited the highest number of dead cells and the strongest ROS signal.Conclusion ZrMOF-Fc NPs show good biosafety and efficient microwave thermal-dynamic conversion capability,and can significantly kill Cal-27 cells.