新斑型黄缘巧瓢虫线粒体基因组全序列测定与分析OA
Complete sequence detection and analysis on mitochondrial genome of new spot-pattern morph of Oenopia sauzeti(Coleoptera:Coccinellidae)
[目的]探究新斑型黄缘巧瓢虫(Oenopia sauzeti)的线粒体基因组结构特征,解析其系统发育关系,为天敌昆虫黄缘巧瓢虫的遗传多样性研究和应用提供基础信息.[方法]采用Illumina高通量测序平台对新斑型黄缘巧瓢虫的线粒体基因组进行测序,完成基因注释并进行生物信息学分析,基于13个蛋白编码基因(PCGs),采用最大似然法构建系统发育树,明确其在瓢虫科中的系统位置.[结果]新斑型黄缘巧瓢虫线粒体基因组全长共20394 bp,包含2个rRNA基因、22个tRNA基因、13个PCGs和2个非编码控制区,其中有6个基因间隔区和11个基因重叠区.新斑型黄缘巧瓢虫线粒体基因组A含量为38.58%,T含量为40.67%,C含量为9.05%,G含量为11.70%,GC含量20.75%,AT含量为79.25%,呈现明显的AT偏斜.13个PCGs中,除COX1基因以TTG为起始密码子外,其余基因均以ATN(ATA、ATG、ATT)为起始密码子;终止密码子方面,COX1、COX2、COX3和ND6基因均以不完整的T为终止密码子,CYTB基因以完整的TAG为终止密码子,其余基因均以完整的TAA为终止密码子.22个tRNA基因中,除trnS1基因呈现昆虫线粒体mt-tRNA典型的D臂缺失,其余21个tRNA基因均呈现典型的三叶草二级结构.系统发育分析结果显示,新斑型Oenopia sauzeti new与已知的黄缘巧瓢虫聚在同一高支持率(100%)分支中,从分子序列上有力证明该新斑型属于黄缘巧瓢虫;六斑异瓢虫为黄缘巧瓢虫和六星瓢虫(Oenopia formosana)的姐妹群.[结论]新斑型黄缘巧瓢虫的全线粒体基因组序列与结构及其一般瓢虫科昆虫线粒体基因组特征类似,且与六星瓢虫和六斑异瓢虫的亲缘关系较近.
[Objective]To investigate the structural characteristics of the mitochondrial genome of new spot-pattern morph of Oenopia sauzeti,and to analyze its phylogenetic relationships,thereby providing fundamental information for the study and application of the genetic diversity of this natural enemy insect.[Method]The mitochondrial genome of new spot-pattern morph of Oenopia sauzeti was sequenced using the Illumina high-throughput sequencing platform.Gene an-notation and bioinformatics analysis were performed.Based on 13 protein-coding genes(PCGs),a phylogenetic tree was constructed using the maximum likelihood method to clarify its phylogenetic position within the family Coccinellidae.[Result]The complete mitogenome of the new spot-pattern morph of Oenopia sauzeti was 20394 bp in length,comprising 2 rRNA genes,22 tRNA genes,13 PCGs,and 2 non-coding control regions.It contained 6 intergenic spacers and 11 gene overlaps.The nucleotide composition of new spot-pattern morph of Oenopia sauzeti was A content=38.58%,T con-tent=40.67%,C content=9.05%,and G content=11.70%,with GC content of 20.75%and AT content of 79.25%,indi-cating a pronounced AT bias.Among the 13 PCGs,COX1 gene initiated with the codon TTG,while the remaining genes initiated with codon ATN(ATA,ATG,or ATT).Regarding stop codons,COX1,COX2,COX3,and ND6 gens termi-nated with an incomplete T;CYTB gene terminated with the complete TAG;and the remaining genes terminated with the complete TAA.Among the 22 tRNA genes,21 exhibited the typical cloverleaf secondary structure,except for trnS1 gene,which lacked the dihydrouridine(D)arm,a characteristic feature of insect mitochondrion mt-tRNAs.Phylogenetic analysis revealed that the new spot-pattern morph of Oenopia sauzeti new clustered within a highly supported clade(100%)alongside the known Oenopia sauzeti,strongly confirming at the molecular level that this new spot-pattern morph belonged to Oenopia sauzeti.Furthermore,Aiolocaria hexaspilota was identified as the sister group to Oenopia sauzeti and Oenopia formosana.[Conclusion]The complete mitogenome sequence and structure of the new spot-pattern morph of Oenopia sauzeti are consistent with the general characteristics of coccinellid mitochondrial genomes.Oenopia sauzeti exhibits a close phylogenetic relationship with Oenopia formosana and Aiolocaria hexaspilota.
陈浩帆;何星;李亚金;赵林;张利娟;张宏瑞
云南农业大学植物保护学院,云南 昆明 650201云南农业大学植物保护学院,云南 昆明 650201云南农业大学植物保护学院,云南 昆明 650201曲靖市植保植检站,云南 曲靖 655000河南农业大学植物保护学院,河南 郑州 450002云南农业大学植物保护学院,云南 昆明 650201
农业科技
新斑型黄缘巧瓢虫线粒体基因组系统发育
new spot-pattern morph of Oenopia sauzetimitochondriongenomephylogenetic relationship
《南方农业学报》 2026 (7)
1957-1966,10
云南省科技厅科技计划项目(202304BT090032)华坪县农民院士科技服务站项目(云科协联[2023]9号) Science and Technology Plan Project of Yunnan Department of Science and Technology(202304BT090032)Sci-tech Service Station of Farmer Academician in Huaping(Yunkexielian[2023]9)
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