白菜黑斑病人工接种鉴定体系的建立以及种质资源筛选OA

Black spot disease is a major fungal disease threatening Chinese cabbage production worldwide.To establish a reliable,reproducible,and efficient phenotyping system to evaluate seedling resistance to black spot disease caused by Alternaria brassicicola,we systematically optimized the inoculation conditions by comparing inoculation methods,spore suspension concentrations,and incubation temperatures.The results showed that detached-leaf inoculation performed on seedlings with a spore suspension of 1×105 spores·mL-1,followed by incubation at 25℃for 3 days,provided the most effective and repeatable conditions for assessing black spot disease resistance.The optimized assay was further validated using 53 elite Chinese cabbage inbred lines,with parallel field disease assessments conducted at the adult plant stage.The high concordance between the two evaluation approaches verified the accuracy,stability,and reliability of the artificial inoculation system.Furthermore,germplasm lines representing the extremes of disease resistance were selected,providing valuable parental materials for identifying genes associated with black spot disease resistance.Together,these results provide both germplasm resources and a technical foundation to advance molecular breeding for enhanced black spot disease resistance in Chinese cabbage.