广西部分地区鸽源鼠伤寒沙门菌的分离鉴定、耐药性及致病性研究OA

To investigate the Salmonella serotypes prevalent in meat pigeon farms in some regions of Guangxi,as well as their antimicrobial resistance,pathogenicity,and associated resistance and virulence genes,22 liver tissue samples were collected from dead squabs suspected of Salmonella infection for pathogen isolation and identification.Antimicrobial susceptibility testing,resistance gene detection,virulence gene detection,and animal regression experiments were subsequently performed.The results showed that 22 strains of Salmonella typhimurium were isolated and identified from the 22 liver tissue samples.Antimicrobial susceptibility testing revealed that the isolates showed the highest resistance rates to tetracycline and doxycycline,both reaching 81.8%(18/22).Resistance gene detection showed that the isolates carried the β-lactam resistance gene blaTEM(100%,22/22),the sulfonamide resistance gene sul2(63.6%,14/22),and the tetracycline resistance gene tet(A)(77.3%,17/22).Virulence gene detection demonstrated that all isolates carried 12 virulence genes,with detection rates of 100%(22/22)for each gene.Animal regression experiments showed that the isolates caused mortality in meat pigeons after infection,and polymerase chain reaction(PCR)confirmed that the isolates were Salmonella typhimurium.These results indicate that Salmonella typhimurium is the dominant serotype circulating in pigeon flocks in some regions of Guangxi.The isolates exhibited the highest resistance to tetracycline antibiotics,carried multiple antimicrobial resistance and virulence genes,and showed strong pathogenicity,providing a reference for the prevention and control of pigeon salmonellosis.