RBPJ对鸭胚成纤维细胞增殖和基因表达的影响机制OA

During the egg-laying process,the oviduct of laying ducks must continuously undergo tissue remodeling,cell renewal,and homeostasis maintenance.Among these processes,fibroblasts,as the primary stromal cells,play a fundamental role in extracellular matrix remodeling,supporting epithelial differentiation,and maintaining smooth muscle function.The recombination signal binding protein for immunoglobulin kappa J region(RBPJ)is an impor-tant effector of the Notch signaling pathway,which regulates cell proliferation and differentiation,thereby affecting tissue development,morphology,and function.However,whether RBPJ regulates the function of duck oviduct by in-fluencing duck oviduct fibroblasts has not yet been reported.This study investigated the effects of RBPJ on the viabil-ity and proliferation of duck embryo fibroblasts and its regulatory role in downstream gene expression through cell cul-ture,RNA interference(RNAi),and transcriptome analysis.The results showed that:(1)Duck embryo fibroblasts were successfully isolated and RBPJ-interfered cell models were successfully constructed;(2)RBPJ gene interfer-ence significantly(p<0.05)reduced the viability at 12 h and the proliferation ability at 24 h of duck embryonic fi-broblasts;(3)After RBPJ gene interference,364 genes were differentially expressed and these significantly enriched in signaling pathways such as the MAPK and calcium signaling pathways.In conclusion:RBPJ affects the MAPK signaling pathway by regulating the expression of the PLA2G4C and AREG genes,and affects the calcium signaling pathway by regulating the expression of the FGF9,NTRK3,and AGTR1 genes,thereby affecting cell proliferation.These findings fill the research gap regarding the role of RBPJ in the proliferation of duck embryo fibroblasts.Moreo-ver,they lay a research foundation for future analysis of RBPJ functions in duck oviduct fibroblasts and provide a the-oretical basis and scientific support for improving egg-laying performance in practical production.