碟花金丝桃xanthones类成分及其抑菌活性研究OA

AIM To study the xanthones from Hypericum addingtonii N.Robson and their antibacterial activity.METHODS The 90％ethanol extract was isolated and purified by silica gel,Sephadex LH-20,ODS and preparative HPLC,then and the structures of the obtained compounds were identified by physicochemical properties and spectral data.The microdilution method was applied to evaluate the antibacterial activity.RESULTS Fifteen xanthones were isolated and identified as hyperxanthone E(1),5-hydroxy-2-methoxyxanthone(2),2-hydroxyxanthone(3),6-O-methyl-2-deprenylrheediaxanthone B(4),toxyloxanthone B(5),1,7-dihydroxyxanthone(6),2-methoxy-3-hydroxyxanthone(7),2,3-dimethoxyxanthone(8),1,5-dihydroxy-6,7-dimethoxyxanthone(9),1,3,5-trihydroxyxanthone(10),1,3,7-trihydroxyxanthone(11),1,7-dihydroxy-3-methoxyxanthone(12),3-methoxy-5'-demethoxycadesin G(13),1,5-dihydroxy-2-methoxyxanthone(14)and 1,7-dihydroxy-6-methoxyxanthone(15).The MIC values of compounds 1-15 against Chromobacterium violaceum ATCC12472 and Pseudomonas aeruginosa were all greater than 500 μg/mL.Among them,at a mass concentration of 250 μg/mL,the inhibition rates of compounds 1,7,10 and 13 on the pigment production of C.violaceum were 88.35％±3.05％,81.61％±2.67％,93.04％±4.20％and 84.51％±3.11％,respectively.CONCLUSION Compounds 1-15 are isolated from H.addingtonii for the first time.Compounds 1,7,10 and 13 exhibit certain inhibitory activity on the pigment production of C.violaceum.