辣椒根际合成菌群的构建及其促生效果评价OA
Construction of a synthetic community from the pepper rhizosphere and evaluation of its growth-promoting effects
辣椒(Capsicum annuum L.)生产长期受到过量施肥与土传病害的双重制约.传统化学改良剂虽常用于缓解这类问题,但长期施用易引起土壤微生态失衡及理化性质退化等次生问题.鉴于此,本研究构建了辣椒根际功能型合成菌群,并对其促生效果进行评价,旨在为该菌群在辣椒绿色生产中的应用提供理论依据.本研究以实验室前期从辣椒根际分离鉴定的12 株具有固氮、溶磷、解钾及产吲哚乙酸等促生特性的植物根际促生菌为基础,基于功能互补原则,从中筛选并构建了4 个合成菌群(Syncom 1~4),并通过盆栽试验系统评价了其对辣椒生长的促进作用.结果表明,Syncom 4 表现出最优的促生效果.与单菌处理相比,Syncom 4形成生物膜的能力显著(p<0.01 或p<0.001)增强,且菌株间无拮抗作用.该菌群促进了辣椒幼苗根毛发育,提高了植株生物量.此外,Syncom 4 显著降低了根际土壤pH值和有效钾含量.16S rRNA扩增子测序结果显示,Syncom 4 处理改变了辣椒根内及根际细菌的群落结构,并在辣椒根内和根际中富集了一些通常与植物促生和生物防治相关的菌属,如假单胞菌属(Pseudomonas)、肠杆菌属(Enterobacter)、芽孢杆菌属(Bacil-lus)、黄杆菌属(Flavobacterium)和微杆菌属(Microbacterium)等.综上,Syncom 4 对辣椒幼苗具有良好的促生效果,为辣椒复合微生物肥料的开发提供了菌种资源与技术支持.
Excessive fertilization and soil-borne diseases have long restricted the production of pepper(Capsicum an-nuum L.).Traditional chemical amendments,though commonly used to alleviate these constraints,tend to cause secondary problems such as soil microecological imbalance and degradation of physicochemical properties after long-term application.In light of this,the present study constructed a functional synthetic microbial community from the pepper rhizosphere and evaluated its growth-promoting effects,aiming to provide a theoretical basis for its application in sustainable pepper production.Building upon 12 strains of plant growth-promoting rhizobacteria(PGPR)previ-ously isolated and identified from the pepper rhizosphere in laboratory studies,strains exhibiting nitrogen-fixing,phosphorus-solubilizing,potassium-releasing,and indole-3-acetic acid(IAA)-producing properties were selected,and four synthetic microbial communities(Syncom 1-4)were constructed based on principles of functional comple-mentarity.Their growth-promoting effects on pepper were systematically evaluated through pot experiments.The re-sults indicated that Syncom 4 exhibited optimal growth-promoting effects.Compared with single-strain treatments,Syncom 4 demonstrated a significant(p<0.01 or p<0.001)enhanced capacity for biofilm formation,with no ob-served inter-strain antagonism.This consortium significantly promoted root hair development in pepper seedlings and increased plant biomass.Furthermore,Syncom 4 significantly reduced the pH level and available potassium content inrhizosphere soil.16S rRNA amplicon sequencing revealed that Syncom 4 treatment altered bacterial community structures within pepper roots and the rhizosphere,enriched certain beneficial bacterial taxa,such as Pseudomonas,Enterobacter,Bacillus,Flavobacterium,and Microbacterium.In summary,Syncom 4 exhibits excellent growth-pro-moting effects on pepper seedlings,providing microbial strain resources and technical support for the development of composite microbial fertilisers for peppers.
姜贵武;张琳;徐伟慧;王志刚
齐齐哈尔大学 生命科学与农林学院,黑龙江省农业微生物制剂产业化工程技术研究中心,黑龙江省农用生物制剂产业化协同创新中心,黑龙江省高浓度有机废液生物转化及产业化概念验证中心,黑龙江 齐齐哈尔 161006齐齐哈尔大学 生命科学与农林学院,黑龙江省农业微生物制剂产业化工程技术研究中心,黑龙江省农用生物制剂产业化协同创新中心,黑龙江省高浓度有机废液生物转化及产业化概念验证中心,黑龙江 齐齐哈尔 161006齐齐哈尔大学 生命科学与农林学院,黑龙江省农业微生物制剂产业化工程技术研究中心,黑龙江省农用生物制剂产业化协同创新中心,黑龙江省高浓度有机废液生物转化及产业化概念验证中心,黑龙江 齐齐哈尔 161006齐齐哈尔大学 生命科学与农林学院,黑龙江省农业微生物制剂产业化工程技术研究中心,黑龙江省农用生物制剂产业化协同创新中心,黑龙江省高浓度有机废液生物转化及产业化概念验证中心,黑龙江 齐齐哈尔 161006
农业科技
辣椒根际促生菌合成菌群促生效果
Capsicum annuum L.plant growth promoting rhizobacteriasynthetic bacterial communitygrowth-promoting effect
《浙江农业学报》 2026 (7)
1366-1377,12
黑龙江省重点研发计划(GA23B018)中国科学院科研项目
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