牛骨骼肌细胞-肌内脂肪细胞互作中关键circRNAs筛选及ceRNA网络解析OA

To investigate the effect of bovine skeletal muscle cells on the circular RNA(circRNA)expression profile in intramuscular adipocytes under co-culture conditions,and to elucidate the potential regulatory mechanisms of circRNA in intramuscular fat deposition,this study established a Transwell co-culture model of bovine skeletal muscle cells and intramuscular adipocytes.Meanwhile,circRNA sequencing was performed on intramuscular adipocytes cultured separately as the control group and those co-cultured with skeletal muscle cells as the experimental group.Differential expression analysis was conducted using edgeR software,with a threshold of llog2FCI≥1 andP＜0.05 to identify differentially expressed circRNAs.GO and KEGG enrichment analyses were performed for the host genes of these circRNAs.A circRNA-miRNA-mRNA ceRNA regulatory network was constructed using the OmicShare online platform to further screen key regulatory factors.The results showed that:1)A total of 10 081 circRNAs were identified,including 3 881 unique to the control group,3 465 unique to the co-culture group,and 2 735 shared by both groups.2)A total of 51 differentially expressed circRNAs were identified,including 16 upregulated and 35 downregulated in the co-culture group compared to the control group.3)The differentially expressed circRNAs derived genes were mainly enriched in GO terms such as histone methyltransferase activity and signaling pathways such as ECM-receptor interaction.4)A total of 42 potential ceRNA regulatory pairs were identified,with novel_circ_002953 occupying a central position in the network.5)Novel_circ_002953 may affect the expression of adipogenic differentiation-related genes such as PSAP and DHCR24 by sponging novel-m0002-3p.Novel_circ_002953 and novel_circ_006185 may regulate the expression of lipid metabolism key genes,including WNT5A and ZBTB16,by sponging novel-m0108-3p.In summary,the co-culture environment can alter the circRNA expression profile of bovine intramuscular adipocytes.This study provides novel candidate circRNA molecules for dissecting the skeletal muscle-adipose interaction regulatory network.