高效液相色谱法同时测定龙丹丸中11种成分含量OA

Objective To establish a high-performance liquid chromatography(HPLC)method for the simultaneous content determination of 11 ingredients in Longdan Pills.Methods The chromatographic column was Stamsil-BP C18 column(250 mm×4.6 mm,5 µm),the mobile phase was 0.1%phosphoric acid-acetonitrile(gradient elution),the flow rate was 0.8 mL/min,the detection wavelength was 230 nm(albiflorin,paeoniflorin)and 280 nm(danshensu,cryptochlorogenic acid,gentiopicroside,isochlorogenic acid B,apigenin-7-O-glucoside,baicalin,salvianolic acid B,wogonoside,baicalein),the column temperature was 30℃,and the injection volume was 5 µL.Results The linear ranges of the above 11 ingredients were 68.07-1 021.87 µg/mL,57.04-855.53 µg/mL,18.66-280.74 µg/mL,1.03-15.67 µg/mL,58.01-870.14 µg/mL,6.31-93.93 µg/mL,6.14-91.68 µg/mL,143.53-2 152.54 µg/mL,49.44-740.84 µg/mL,38.27-574.11 µg/mL,3.03-45.44 µg/mL,respectively.The limits of detection were 0.098 0-12.993 2 µg/mL,and the limits of quantification were 0.326 8-43.310 6 µg/mL.The RSDs of precision,stability and repeatability test results were all lower than 3.0%.The average recoveries were 92.34%-102.40%,with RSDs of 1.52%-4.46%(n=6).Conclusion The established method is sample,accurate,sensitive,reproducible,and specific,and can be used for the quality control of Longdan Pills.