MF63与MK886在大肠杆菌诱导奶牛子宫内膜炎中的潜在治疗作用OA

Endometritis in dairy cows is a common reproductive disease caused by pathogenic bacte-rial infection.Its occurrence and progression are closely associated with an imbalance in the inflam-matory response and abnormalities in prostaglandin-mediated immune regulation.This study aims to evaluate the regulatory effects of the microsomal prostaglandin E synthase-1 inhibitor MF63 and the 5-lipoxygenase-activating protein inhibitor MK886 on inflammatory responses in bovine macrophages and endometrial tissues induced with Escherichia coli(E.col i).Results demonstrated that MF63 and MK886 significantly suppress the secretion of proinflammatory cytokine IL-6 and chemokine IL-8 induced by E.coli,while simultaneously upregulating the secretion of anti-inflammatory factor IL-10(P＜0.05).This suggests they exhibit pronounced anti-inflammatory regulatory effects.Additionally,both in-hibitors significantly reduced the expression of HMGB-1 and HABP-2 in infected cells and endometrial tissue(P＜0.01)and attenuated apoptosis.In summary,MF63 and MK886 attenuate the inflammatory re-sponse and tissue damage induced by E.coli infection by modulating the balance between pro-inflammato-ry and anti-inflammatory mediators,thereby providing potential therapeutic targets for the preven-tion and treatment of bovine endometritis.