香草酸对猪卵母细胞体外成熟及受精能力的影响OA

The aim of this study was to investigate the regulatory effects of vanillic acid on in vitro maturation,fertilization ability and embryonic development of porcine oocytes,and to clarify its protective effect on oxidative stress-damaged oocytes.The cumulus-oocyte complexs was used as the research object,and the blank control group and 0,5,10,25,50,100 μmol/L vanillic acid treatment groups were set up,the germinal vesicle rupture(GVBD)rate,MⅡ maturation rate and apoptosis rate of oocytes were detected,and the protective effect of 25 μmol/L vanillic acid on damaged porcine oocytes was analyzed by using 1 mmol/L H2O2-induced oxidative damage model The fertilization rate of oocytes,the monospermic fertilization rate,the cleavage rate of early embryos,the blastocyst formation rate,the total cell number of blastocysts and the apoptosis rate of early blastocysts were further deter-mined after treatment with different concentrations of vanillic acid.The results showed that differ-ent concentrations of vanillic acid had no significant effect on the incidence of GVBD in porcine oocytes.25 μmol/L vanillic acid could significantly increase the maturation rate of MⅡ stage and re-duce the apoptosis rate(P＜0.05).In addition,the inhibitory effect of vanillic acid on oocyte apopto-sis was concentration-dependent.10-25 μmol/L vanillic acid could significantly improve the fertil-ization rate of oocytes(P＜0.05),and the effect of 25 μmol/L group was the best.The monospermic fer-tilization rate of 100 μmol/L group was significantly higher than that of the control group(P＜0.05).Vanillic acid had no significant effect on the cleavage rate of embryos,but the blastocyst formation rate in the 10-25 μmol/L group was significantly increased(P＜0.05),and the total number of cells in the 25 μmol/L group was significantly higher than that in the 0 μmol/L and 50 μmol/L groups(P＜0.05),the rate of early blastocyst apoptosis in the 10-100 μmol/L group was significantly lower than that in the control group(P＜0.05).25 μmol/L vanillic acid could effectively alleviate the oxidative stress injury induced by H2O2,significantly increase the MⅡ-stage maturation rate of damaged oocytes and re-duce the apoptosis rate(P＜0.05).This study confirmed that 25 μmol/Lwas the appropriate concentration of vanillic acid to regulate the development of porcine oocytes.It can promote in vitro maturation of porcine oocytes,improve fertilization efficiency and embryo quality of early embryo development through antioxidant and anti-apoptotic effects,and has a significant protective effect on oxidative stress-damaged oocytes.This study provides an important theoretical basis and experimental support for optimizing the in vitro culture system of porcine oocytes and promoting the industrial application of animal embryo engineering.