猫杯状病毒VP1-CDE蛋白单链抗体的筛选与鉴定OA

To develop novel diagnostic and therapeutic tools against feline calicivirus(FCV),phage display technology was employed to screen single-chain fragments variable(scFvs)that specifically target the conserved CDE region of capsid protein VP1(VP1-CDE)of FCV.First,the VP1-CDE protein was produced through recombinant expression and used to immunize SPF chickens,followed by the construc-tion of a chicken-derived scFv phage display library.After three rounds of biopanning,three highly specific scFvs,designated P2D5,P2A7,and PlG9,were successfully obtained.ELISA and Western-blot analyses demonstrated that these scFvs specifically recognized the recombinant VP1-CDE protein with-out cross-reactivity to unrelated proteins.Furthermore,indirect immunofluorescence assays confirmed their specific binding ability to FCV antigens.In conclusion,this study successfully screened scFv antibodies targeting the VP1-CDE region of FCV using phage display technology.The obtained scFvs exhibited high specificity and provided candidate molecules for the development of FCV diagnostic anti-bodies,thereby laying an important experimental foundation for future research.