萍蓬草基因组大小的分析OA

To explore the genome size and characteristics of Nuphar pumila,this study selected 3 N.pumila samples,numbered KMPPC01,KMPPC02,and KMPPC03 for flow cytometry detection.The KMPPC01 was se-lected for high-throughput sequencing.Combined with bioinformatics methods such as k-mer analysis,prelimin-ary research was conducted on the genome of N.pumila.The results are as follows:The genome size of N.pum-ila was determined to be approximately 2 187.12 Mb by flow cytometry and the converted C value was approxim-ately 1.12 pg;the raw data obtained by high-throughput sequencing was 111.76 Gb,and the clean data obtained after filtering was 108.39 Gb;K-mer analysis showed that the genome size of N.pumila was 2094.71 Mb,the GC content was 38.20％,the heterozygosity was 1.89％,the proportion of repeated sequences was 62.63％.In sum-mary,the genome of N.pumila is a highly heterozygous and highly repetitive complex genome with a size of ap-proximately 2094.71-2187.12 Mb.