杨梅素抑制非洲猪瘟病毒感染猪肺泡巨噬细胞作用的鉴定OA

African swine fever(ASF),caused by the African swine fever virus(ASFV),has inflicted substantial losses on the swine industry in our country.Flavonoids exhibit broad-spectrum antiviral activities.Myricetin,a type of flavonoid,has demonstrated antiviral activities against various RNA/DNA viruses.This study evaluates the role of myricetin against genotype II ASFV infectivity to porcine alveolar macrophages(PAMs).During the infection of PAMs by ASFV,which were treated with varying concentrations of myricetin.The impacts of myricetin on the infectivity of ASFV to PAMs were evaluated using methods such as real-time quantitative PCR,Western blotting,immunofluorescence,and hemadsorption assays.Myricetin exhibits minimal toxicity to PAMs,with a CC50 value of(152.270±1.091)μmol·L-1;Myricetin can significantly reduce the viral yield of ASFV,with an IC50 value of(31.670±1.489)μmol·L-1,and it markedly inhibits the transcription of the viral early gene CP204L and the expression of its encoded protein p30;The inhibitory effect is most significant when myricetin is used to pretreat the virus and when added during the virus invasion of PAMs,whereas pretreatment of PAMs with myricetin and addition of myricetin post ASFV infection of PAMs have no significant impact on the viral yield of ASFV;Further results show that myricetin significantly inhibits the transcription of the viral early gene CP204L and the expression of its encoded protein p30 during the adhesion and internalization of ASFV into PAMs.This study is the first to reveal that myricetin exhibits a certain inhibitory effect on ASFV infection in PAMs.