云南人参果花叶病病原物鉴定及遗传进化研究OA

[Objective]The study focused on two representative ecological zones planted with the spring-cropping region(Shilin)and the winter-cropping region(Zhenyuan).It systematically conducted pathogen identification,distribution analysis,and genetic evolution research on S.muricatum mosaic disease.The aim was to clarify the types of pathogenic viruses and their patterns of compound infection,thereby provi-ding theoretical support and a data foundation for overcoming the bottlenecks imposed by viral diseases on industrial development.[Method]Investigation and statistics on the occurrence of mosaic virus disease in the S.muricatum planting areas of Shilin and Zhenyuan,the types of mosaic viruses were detected using electron microscopy,enzyme-linked immunosorbent assay(ELISA),and reverse transcription polymerase chain reaction(RT-PCR).The genetic evolutionary relationships of the mosaic viruses were clarified by constructing phylogenetic trees.[Re-sult]Field investigations revealed that the average disease incidence in the winter planting area(91.3％)was significantly higher than that in the spring planting area(60.9％),with disease indices of 47.7 and 47.56,respectively.ELISA and RT-PCR detection conclusively de-termined that Tomato mosaic virus(ToMV)was the dominant pathogen,with a detection rate of 100.00％in both regions.Cucumber mosaic virus(CMV)was only found in the spring cropping area,with a detection rate of 46.67％,and it consistently co-infected with ToMV.Tobac-co mosaic virus(TMV)was not detected,and the previously positive TMV results in ELISA were attributed to serological cross-reactions with ToMV.Sequence analysis revealed that the ToMV-YN isolate(ID:3019395)exhibited high homology(99.45％-99.82％)with both do-mestic and international strains,indicating genetic conservation.In contrast,the CMV-YN isolate(ID:3019384)showed homology ranging from 95.74％to 99.54％with other isolates from different domestic hosts,demonstrating higher diversity.[Conclusion]The study elucidated the viral composition patterns associated with mosaic disease in Yunnan S.muricatum,identifying ToMV as the primary pathogen and CMV as a regional co-pathogen,thereby providing a scientific basis for targeted detection and control strategies.