木耳粉色色素的结构特性和抗氧化活性OA

[Objective]The study investigates the chromophoric compounds of'Fener No.1',a new cultivar of Auricularia cornea developed by the Sichuan Institute of Edible Fungi,and evaluates the thermal,light,and pH stability as well as the in vitro antioxidant activity of its pink pigment.[Method]Firstly,the pink pigments were extracted by petroleum ether,ethyl acetate,acetone,ethanol,methanol,and water,then,purified using AB-8 macroporous resin.The metabolites in pink pigments were analyzed using untargeted metabolomics to explore the potential coloring substances.Meanwhile,the stability and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS),1,1-diphenyl-2-picryl-hydrazyl(DPPH)radical scavenging activities,the ferric reducing antioxidant power(FRAP)of the pink pigments were also investigated.[Result]The pink pigments could be effectively extracted from A.cornea by 80％ethanol aqueous solution.The absorbance of the purified pigments at 550 nm were significantly enhanced,and the abundance of some metabolites which possessed conjugated systems were significant-ly up-regulated,including indoles,quinones,phenylpropanoids,polyketides and polyphenols.Meanwhile,the abundance of lipids and peptides were also up-regulated,suggesting that the color of A.cornea may be resulted from the synergistic effects of these substances.In addition,the pigments could remain color-stable when the temperature was below 80 ℃ and under neutral or slightly acidic pH conditions.And the optimal storage conditions of the pigments are 4℃ in the dark.Furthermore,the purified pigments exhibited significantly enhanced in vitro antioxi-dant activities,which may be related to the enrichment of metabolites such as quinones,polyketides and polyphenols.[Conclusion]The pink of'Fener No.1'may be attributed to the compounds such as indoles,quinones,phenylpropanoids,polyketides and polyphenols.The pig-ments exhibit favorable thermal stability and should be stored at low-temperature,dark conditions at neutral or slightly acidic pH.Moreover,it exhibits notable in vitro antioxidant activities.The study may provide a theoretical basis for the breeding and application of high-value Auric-ularia varieties.