联合分析揭示成熟芝麻蒴果在闭蒴/裂蒴过程中脂类代谢和转运蛋白调控机制OA

[Objective]The paper aimed to dissect the regulatory network underlying the traits of mature sesame pod closure or splitting at the molecular and metabolic levels,providing new clues and exploration directions for the breeding of sesame varieties with pod closure traits.[Method]Our previous research suggested that lignin might be an important metabolite affecting the closure of sesame capsules.As the core framework of the cell wall,lignin laid the'hardware'foundation for the phenotype from the physical and chemical perspective.However,the functioning of the hardware must rely on the'software'control.Therefore,this time we re-examined the same batch of data and shifted the research dimension.Based on transcriptome sequencing and broad-target metabolite detection,we conducted data mining and research through classification,enrichment,and combined analysis.[Result]GO showed that DEGs were significantly enriched not only in items such as mem-brane transport and microtubule motility,but also in items such as phosphatase activity and ATPase activity.KEGG analysis revealed that the DEGs were enriched in the ABC transporter pathway.The main function of this protein was to utilize the energy generated from ATP hydroly-sis to transport substrates across the membrane in an anti-concentration gradient manner.Moreover,all the DEGs in this pathway were includ-ed in the above-mentioned GO-phosphatase activity and ATPase activity-related entries.DAMs were enriched in the phosphatidylinositol sig-naling system pathway and the inositol phosphate metabolism pathway.The only DAMs shared by both pathways-inositol 1,4-bisphosphate-underwent a simple enzymatic reaction to produce the compound inositol,and it was also involved in the ABC transporter metabolism path-way.DEGs and DAMs were jointly enriched in the fatty acid breakdown pathway,and fatty acid metabolism was a key process in cellular en-ergy metabolism(ATP).Other lipid metabolic pathways also showed enrichment.Furthermore,based on the results of GO enrichment in mul-tiple microtubule-related entries,and by inferring from the transcription factor families to which the DEGs in the entries belong,it could be concluded that the Kinesin-driven protein was also actively involved in the closure and dehiscence of sesame capsules.[Conclusion]Based on the research results,we speculate that during the process of sesame seed capsule closure and rupture,lipid metabolism generates ATP which combines with transport proteins such as ABC or Kinesin,providing energy to facilitate the transport of substrates across the cell mem-brane or within microtubules,thus offering new inspiration and direction for further exploration of the molecular mechanism underlying the closure of sesame capsule at the mature stage.