圆齿野鸦椿WRKY基因家族鉴定及在花果发育中的表达分析OA

[Objective]The paper aimed to further elucidate the regulatory mechanisms and molecular mechanisms of the WRKY gene family in the floral and fruit development of Euscaphis konishii Hayata.[Method]Based on whole-genome sequencing data of the species,the study systematically identified all members of the WRKY transcription factor gene family and conducted a comprehensive analysis of key biological indicators,including their physicochemical properties,conserved motifs,gene structure,phylogenetic relationships,chromosomal distribution and cis-acting elements.[Result]A total of 81 E.konishii WRKY genes were identified,distributed unevenly across 12 chromosomes.Based on the phylogenetic tree,they could be classified into three main groups:Group Ⅰ,Group Ⅱ and Group Ⅲ.Analysis of physicochemical proper-ties revealed that the amino acid count ranged from 120 to 764;hydrophobicity scores were all negative,confirming that all EkWRKY proteins were hydrophilic,and all 81 EkWRKY proteins were localized in the cell nucleus.Prediction of promoter cis-acting elements revealed that the promoter regions of EkWRKY genes contained a large number of elements associated with the regulation of plant growth and development,hor-mone signalling responses,and tolerance to abiotic stress,as well as specific elements related to the flavonoid biosynthesis pathway.Analysis of flower and fruit-specific expression showed that EkWRKY14,EkWRKY09 and EkWRKY38 were highly expressed in petals and stamens,sug-gesting their involvement in the regulation of the flowering process;EkWRKY79,EkWRKY29,EkWRKY09,EkWRKY38 and EkWRKY37 exhib-ited a high-low-high expression trend during fruit development,peaking during the fruit abscission stage.This expression pattern was consist-ent with the unique phenotype of E.konishii,characterized by fruit reddening and maturation,followed by fruit retention for over 130 days af-ter dehiscence without abscission.[Conclusion]The study systematically analyzes the expression characteristics of the EkWRKY gene in the floral and fruit tissues of E.konishii,clarifying the tissue-specific expression patterns of this gene.It provides an important theoretical basis for elucidating the specific characteristics of floral and fruit development in E.konishii and for further exploring its potential medicinal value.