海藻糖通过下调PAI-1/P53通路减轻博来霉素诱导的肺泡上皮细胞衰老OA

This study aims to explore the effect and underlying mechanism of trehalose on bleomycin-induced the senescence of alveolar epithelial cells.Bleomycin was used to induce senescence in human alveolar epithelial-like A549 cells.The degree of senescence in A549 cells,induced by bleomycin with or without trehalose was determined via Western blot and senescence-associatedβ-galactosidase staining.Trehalose was used in combination with the autophagy inhibitors 3-methyladenine or chlo-roquine,and Western blot was used to detect changes in marker proteins of cellular senescence.Additionally,Western blot was used to determine the effects of trehalose on the plasminogen activator inhibitor-1(PAI-1)/P53 pathway.The results demon-strated that trehalose alleviated the senescence of alveolar epithelial cells,and increased the levels of LC3Ⅱ in alveolar epithe-lial cells.Inhibition of autophagy did not reverse the trehalose-induced improvement in alveolar epithelial cell senescence.Trehalose downregulated the expression levels of PAI-1 and P53 in bleomycin-treated alveolar epithelial cells.In conclusion,trehalose alleviated the senescence of alveolar epithelial cells,which was related to the downregulation of PAI-1/P53 pathway but not to autophagy activation induced by trehalose.