红芪蜜炙前后配伍醋莪术对肺癌细胞凋亡、自噬的影响OA

This study aims to compare the effects of honey processed Hedysari Radix-vinegar-processed Curcumae Rhizoma(HPHR-VPCR)and Hedysari Radix-vinegar-processed Curcumae Rhizoma(HR-VPCR)on apoptosis and autophagy in A549 cells and NCI-H1299 cells,and explore the mechanism of HPHR-VPCR inducing apoptosis and autophagy.Twenty-one SPF grade SD rats were randomly divided into three groups:control(Con)group,HR-VPCR group,and HPHR-VPCR group.The dosage of each drug pair was 4.63 g/kg,and the drug containing serum was taken after continuous administration for sev-en days;Hoechst33258 staining was used to detect cell apoptosis morphology,and flow cytometry was used to detect cell apop-tosis rate;The monodansylcadaverine(MDC)method was used to detect cellular autophagy;Transmission electron microscopy was used to detect autophagosomes and autolysosomes;Western blot was used to detect the expression of ubiquitin-binding protein P62(P62),beclin1(BECN1),B-cell lymphoma-2(BCL-2),and BCL-2 associated X protein(BAX)proteins.The results of Hoechst33258 and flow cytometry analysis showed that in the groups treated with HR-VPCR and HPHR-VPCR,chromatin accumulation,nuclear concentration and fragmentation were severe,apoptosis fluorescence was enhanced,and the proportion of apoptosis increased;The MDC method detection results showed that the green fluorescence of the HR-VPCR group and HPHR-VPCR group was enhanced,and autophagosomes were increased;The Western blot results showed that com-pared with the Con group,the expression of P62 and BCL-2 proteins was significantly reduced in HPHR-VPCR group,while the expression of BECN1 and BAX proteins was significantly increased(P＜0.01,P＜0.05).In summary,both HR-VPCR and HPHR-VPCR can induce cell apoptosis and autophagy.HPHR-VPCR is superior to HR-VPCR in inducing cell apoptosis and autophagy.The induction of apoptosis and autophagy in lung cancer cells by HPHR-VPCR is related to the decrease of P62 and BCL-2,as well as the increase of BECN1 and BAX expression.