NtPLA2A介导激素信号通路增强烟草青枯病抗性的机制OA

[Objective]To clone the NtPLA2A gene from tobacco(Nicotiana tabacum)and explore the molecular mechanism by which it mediates hormone signaling pathways to enhance tobacco resistance to bacterial wilt.[Method]This study cloned the NtPLA2A gene from tobacco,conducted bioinformatic analysis and expression profiling under Ralstonia solanacearum infection,and investigated the role of NtPLA2A in disease resistance using virus-induced gene silencing(VIGS)in N.benthamiana.[Result]The NtPLA2A coding sequence is 1 284 bp,encoding 428 amino acids.The protein has a molecular weight of 46.57 kD and a theoretical isoelectric point of 8.74,and is a stable hydrophilic protein.Bioinformatic analysis revealed that NtPLA2A contains a typical Patatin-like phospholipase domain and shares high amino acid sequence similarity with PLA proteins from other species.Promoter analysis indicated that the promoter region of NtPLA2A contains environmental factors such as light,low temperature,and hormones.Following R.solanacearum infection,NtPLA2A was significantly upregulated in both resistant and susceptible tobacco materials to varying degrees.Additionally,treatments with salicylic acid,jasmonic acid,and ethylene also induced its expression.VIGS results demonstrated that silencing NtPLA2A compromised resistance to R.solanacearum in N.benthamiana.[Conclusion]This study clarified the sequence,structure,and expression characteristics of tobacco NtPLA2A gene,which is induced by R.solanacearum and SA/JA/ET;its silencing significantly impairs N.benthamiana resistance to bacterial wilt.