海藻糖-6-磷酸合成酶基因VvTPS10在葡萄耐盐和生长发育中的功能分析OA

[Objective]This study aimed to investigate the response of the VvTPS10 gene to salt stress in grapevine(Vitis vinifera),providing theoretical insights for the study of salt tolerance mechanism in grapevine.[Method]After subjecting potted grape seedlings to salt stress treatment,the expression pattern of VvTPS10 was detected by RT-qPCR.Phenotypic observations of VvTPS10-overexpressing Arabidopsis thaliana and grape calli were performed after salt stress treatment,followed by the determination of physiological and biochemical indices and the analysis of stress-resistant gene expression.[Result]The expression of the VvTPS10 gene in grapevines was significantly up-regulated under salt stress.Under the treatments of 150 mmol/L NaCl and 200 mmol/L NaCl,the germination rates of VvTPS10-overexpressing A.thaliana were 18.04%and 50.57%higher than those of the wild-type,respectively.Under salt stress,the relative electrical conductivity,MDA content and superoxide anion content of VvTPS10-overexpressing A.thaliana were significantly lower than those of the wild-type,whereas the chlorophyll content,and the activities of SOD,POD and CAT were significantly higher than those of the wild-type.RT-qPCR analysis showed that under salt stress,the expression levels of stress-resistant genes including COR15A、COR47、RD29B、SOS1、NHX1 and DREB2A in VvTPS10-overexpressing plants were significantly higher than those in the wild-type.Meanwhile,we found that overexpression of VvTPS10 not only resulted in an early bolting and flowering in A.thaliana but also responded to exogenous sucrose treatment,as evidenced by a significant increase in anthocyanin accumulation.Finally,under salt stress,VvTPS10-overexpressing grape calli exhibited better growth status,higher fresh weight and lower relative electrical conductivity.[Conclusion]The VvTPS10 gene is induced by salt stress,and it enhances the salt tolerance of A.thaliana by maintaining cell membrane stability,improving antioxidant enzyme activities and promoting the expression of stress-resistant genes.In addition,this gene promotes early flowering of A.thaliana and increases anthocyanin accumulation under sucrose treatment.Similarly,overexpression of VvTPS10 enhanced salt tolerance in grape callus.