香蕉枯萎菌侵染香蕉抗/感品种的差异表达lncRNAs及其功能初步研究OA

This study analyzed the functions of high-abundance differentially expressed long noncoding RNAs(DElncRNAs)in Foc TR4 reisolates from resistant and susceptible banana cultivars,aiming to explore the regulatory roles of DElncRNAs in critical biological processes,including fungal growth,development,and pathogenicity.RNA-seq and lncRNA identification were performed on reisolated Foc TR4 strains,and high-abundance DElncRNAs were screened.qRT‒PCR was adopted to analyze the expression correlation between DElncRNAs and their predicted target genes.The split-marker technique was ap-plied to knock out target DElncRNA genes.Subsequently,the colony diameter,spore yield,and pathogenicity of ΔlncRNA knockout mutants were determined and analyzed.The results revealed that 626 DElncRNAs and 9 904 cis-target genes were identified via transcriptome sequencing.Seven high-abundance DElncRNAs,MSTRG.1558.1 and MSTRG.9709.3,were ob-tained.These seven DElncRNAs presented positive or negative expression correlations with thirteen predicted target genes.The ΔlncRNA_1558.1 mutant showed slow growth,pink colony reversal,altered conidial morphology,and decreased spore production.The seven knockout mutants exhibited distinct responses to diverse stress conditions.Six mutants represented attenuated pathogenicity,whereas ΔlncRNA_22894.1 displayed enhanced pathogenicity.It is concluded that the seven DElncRNAs represented by lncRNA_1558.1 participate in modulating the growth,stress response and pathogenic processes of Foc TR4.The findings offer novel targets for clarifying the pathogenic mechanism of the banana Fusarium wilt fungus and breeding disease-resistant banana cultivars.